A new usage of functionalized oligodeoxynucleotide probe for site-specific modification of a guanine base within RNA

Kazumitsu Onizuka, Yosuke Taniguchi, Shigeki Sasaki

研究成果: Contribution to journalArticle査読

19 被引用数 (Scopus)

抄録

Site-specific modification of RNA is of great significance to investigate RNA structure, function and dynamics. Recently, we reported a new method for sequence-and cytosine-selective chemical modification of RNA based on the functional group transfer reaction of the 1-phenyl-2-methylydene-1,3-diketone unit of the 6-thioguanosine base incorporated in the oligodeoxynucleotide probe. In this study, we describe that the functionality transfer rate is greatly enhanced and the selectivity is shifted to the guanine base when the reaction is performed under alkaline conditions. Detailed investigation indicated that the 2-amino group of the enolate form of rG is the reactant of the functionality transfer reaction. As a potential application of this efficient functionality transfer reaction, a pyrene group as a relatively large fluorescent group was successfully transferred to the target guanine base of RNA with a high guanine and site selectivity. This functionality transfer reaction with high efficiency and high site-selectivity would provide a new opportunity as a unique tool for the study of RNA.

本文言語英語
論文番号gkp930
ページ(範囲)1760-1766
ページ数7
ジャーナルNucleic acids research
38
5
DOI
出版ステータス出版済み - 1 29 2010

All Science Journal Classification (ASJC) codes

  • 遺伝学

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