A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein

Hajime Niwa, Yasuhiro Miyauchi-Nanri, Kanji Okumoto, Satoru Mukai, Kentaro Noi, Teru Ogura, Yukio Fujiki

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

A newly isolated binding protein of peroxisomal targeting signal type 2 (PTS2) receptor Pex7, termed P7BP2, is transported into peroxisomes by binding to the longer isoform of Pex5p, Pex5pL, via Pex7p. The binding to Pex7p and peroxisomal localization of P7BP2 depends on the cleavable PTS2 in the N-terminal region, suggesting that P7BP2 is a new PTS2 protein. By search on human database, three AAA+ domains are found in the N-terminal half of P7BP2. Protein sequence alignment and motif search reveal that in the C-terminal region P7BP2 contains additional structural domains featuring weak but sufficient homology to AAA+ domain. P7BP2 behaves as a monomer in gel-filtration chromatography and the single molecule observed under atomic force microscope shapes a disc-like ring. Collectively, these results suggest that P7BP2 is a novel dynein-type AAA+ family protein, of which domains are arranged into a pseudo-hexameric ring structure.

本文言語英語
ページ(範囲)437-447
ページ数11
ジャーナルJournal of biochemistry
164
6
DOI
出版ステータス出版済み - 12 1 2018

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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