A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein

Hajime Niwa, Yasuhiro Miyauchi-Nanri, Kanji Okumoto, Satoru Mukai, Kentaro Noi, Teru Ogura, Yukio Fujiki

研究成果: ジャーナルへの寄稿記事

抄録

A newly isolated binding protein of peroxisomal targeting signal type 2 (PTS2) receptor Pex7, termed P7BP2, is transported into peroxisomes by binding to the longer isoform of Pex5p, Pex5pL, via Pex7p. The binding to Pex7p and peroxisomal localization of P7BP2 depends on the cleavable PTS2 in the N-terminal region, suggesting that P7BP2 is a new PTS2 protein. By search on human database, three AAA+ domains are found in the N-terminal half of P7BP2. Protein sequence alignment and motif search reveal that in the C-terminal region P7BP2 contains additional structural domains featuring weak but sufficient homology to AAA+ domain. P7BP2 behaves as a monomer in gel-filtration chromatography and the single molecule observed under atomic force microscope shapes a disc-like ring. Collectively, these results suggest that P7BP2 is a novel dynein-type AAA+ family protein, of which domains are arranged into a pseudo-hexameric ring structure.

元の言語英語
ページ(範囲)437-447
ページ数11
ジャーナルJournal of Biochemistry
164
発行部数6
DOI
出版物ステータス出版済み - 12 1 2018

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Dyneins
Proteins
Peroxisomes
Sequence Alignment
Protein Transport
Chromatography
Gel Chromatography
Carrier Proteins
Protein Isoforms
Microscopes
Monomers
Gels
Databases
Molecules
peroxisomal targeting signal 2 receptor

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein. / Niwa, Hajime; Miyauchi-Nanri, Yasuhiro; Okumoto, Kanji; Mukai, Satoru; Noi, Kentaro; Ogura, Teru; Fujiki, Yukio.

:: Journal of Biochemistry, 巻 164, 番号 6, 01.12.2018, p. 437-447.

研究成果: ジャーナルへの寄稿記事

Niwa, H, Miyauchi-Nanri, Y, Okumoto, K, Mukai, S, Noi, K, Ogura, T & Fujiki, Y 2018, 'A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein', Journal of Biochemistry, 巻. 164, 番号 6, pp. 437-447. https://doi.org/10.1093/jb/mvy073
Niwa, Hajime ; Miyauchi-Nanri, Yasuhiro ; Okumoto, Kanji ; Mukai, Satoru ; Noi, Kentaro ; Ogura, Teru ; Fujiki, Yukio. / A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein. :: Journal of Biochemistry. 2018 ; 巻 164, 番号 6. pp. 437-447.
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abstract = "A newly isolated binding protein of peroxisomal targeting signal type 2 (PTS2) receptor Pex7, termed P7BP2, is transported into peroxisomes by binding to the longer isoform of Pex5p, Pex5pL, via Pex7p. The binding to Pex7p and peroxisomal localization of P7BP2 depends on the cleavable PTS2 in the N-terminal region, suggesting that P7BP2 is a new PTS2 protein. By search on human database, three AAA+ domains are found in the N-terminal half of P7BP2. Protein sequence alignment and motif search reveal that in the C-terminal region P7BP2 contains additional structural domains featuring weak but sufficient homology to AAA+ domain. P7BP2 behaves as a monomer in gel-filtration chromatography and the single molecule observed under atomic force microscope shapes a disc-like ring. Collectively, these results suggest that P7BP2 is a novel dynein-type AAA+ family protein, of which domains are arranged into a pseudo-hexameric ring structure.",
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AU - Okumoto, Kanji

AU - Mukai, Satoru

AU - Noi, Kentaro

AU - Ogura, Teru

AU - Fujiki, Yukio

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