A noninvasive method for extracting bivalve DNA from the water-filled mantle cavity

Yoshihisa Kurita, Akihiro Kijima

研究成果: ジャーナルへの寄稿記事

抄録

Genetic studies play a great role for determining the biology of bivalves, particularly those covering population genetics, phylogeny, breeding, stock management, and conservation. However, DNA sampling methods that require removal of bivalves from the water and/or opening of their shells often cause stress and damage to bivalves, which can be lethal. The invasiveness of DNA sampling has made it difficult to conduct genetic studies in threatened species, rare species, and/or breeding lineages. In the present study, we developed a non-invasive method for bivalve DNA sampling using the water-filled mantle cavity (WMC). Our method can extract DNA from a small WMC sample (about 100 µl), collected using a fine needle and syringe without opening the shell. We demonstrated that the WMC sample contains intact mitochondrial and nuclear DNA. DNA contamination from other organisms, such as adjacent bivalve individuals, did not affect the resulting PCR and DNA sequencing analyses. Finally, the individuals from whom WMC was collected remained alive for more than 2 months after the experiments. This non-invasive method will be of great assistance in investigating the genetics of bivalves.

元の言語英語
ページ(範囲)237-243
ページ数7
ジャーナルHydrobiologia
829
発行部数1
DOI
出版物ステータス出版済み - 2 1 2019

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bivalve
Bivalvia
cavity
mantle
DNA
shell (molluscs)
water
sampling
methodology
breeding
breeding stock
shell
syringes
invasiveness
threatened species
lethal genes
nuclear genome
method
rare species
population genetics

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

A noninvasive method for extracting bivalve DNA from the water-filled mantle cavity. / Kurita, Yoshihisa; Kijima, Akihiro.

:: Hydrobiologia, 巻 829, 番号 1, 01.02.2019, p. 237-243.

研究成果: ジャーナルへの寄稿記事

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