A novel β-defensin structure: A potential strategy of big defensin for overcoming resistance by gram-positive bacteria

Takahide Kouno, Naoki Fujitani, Mineyuki Mizuguchi, Tsukasa Osaki, Shin Ichiro Nishimura, Shun-Ichiro Kawabata, Tomoyasu Aizawa, Makoto Demura, Katsutoshi Nitta, Keiichi Kawano

研究成果: ジャーナルへの寄稿記事

24 引用 (Scopus)

抄録

Big defensin is a 79-residue peptide derived from hemocytes of the Japanese horseshoe crab. It has antimicrobial activities against Gram-positive and -negative bacteria. The amino acid sequence of big defensin can be divided into an N-terminal hydrophobic half and a C-terminal cationic half. Interestingly, the trypsin cleaves big defensin into two fragments, the N-terminal and C-terminal fragments, which are responsible for antimicrobial activity against Gram-positive and -negative bacteria, respectively. To explore the antimicrobial mechanism of big defensin, we determined the solution structure of mature big defensin and performed a titration experiment with DPC micelles. Big defensin has a novel defensin structure; the C-terminal domain adopts a β-defensin structure, and the N-terminal domain forms a unique globular conformation. It is noteworthy that the hydrophobic N-terminal domain undergoes a conformational change in micelle solution, while the C-terminal domain remains unchanged. Here, we propose that the N-terminal domain achieves its antimicrobial activity in a novel fashion and explain that big defensin has developed a sttategy different from those of other β-defensins to suppress the growth of Gram-positive bacteria.

元の言語英語
ページ(範囲)10611-10619
ページ数9
ジャーナルBiochemistry
47
発行部数40
DOI
出版物ステータス出版済み - 10 7 2008

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Defensins
Gram-Positive Bacteria
Bacteria
Micelles
Gram-Negative Bacteria
Horseshoe Crabs
Hemocytes
Titration
Trypsin
Conformations
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

A novel β-defensin structure : A potential strategy of big defensin for overcoming resistance by gram-positive bacteria. / Kouno, Takahide; Fujitani, Naoki; Mizuguchi, Mineyuki; Osaki, Tsukasa; Nishimura, Shin Ichiro; Kawabata, Shun-Ichiro; Aizawa, Tomoyasu; Demura, Makoto; Nitta, Katsutoshi; Kawano, Keiichi.

:: Biochemistry, 巻 47, 番号 40, 07.10.2008, p. 10611-10619.

研究成果: ジャーナルへの寄稿記事

Kouno, T, Fujitani, N, Mizuguchi, M, Osaki, T, Nishimura, SI, Kawabata, S-I, Aizawa, T, Demura, M, Nitta, K & Kawano, K 2008, 'A novel β-defensin structure: A potential strategy of big defensin for overcoming resistance by gram-positive bacteria', Biochemistry, 巻. 47, 番号 40, pp. 10611-10619. https://doi.org/10.1021/bi800957n
Kouno, Takahide ; Fujitani, Naoki ; Mizuguchi, Mineyuki ; Osaki, Tsukasa ; Nishimura, Shin Ichiro ; Kawabata, Shun-Ichiro ; Aizawa, Tomoyasu ; Demura, Makoto ; Nitta, Katsutoshi ; Kawano, Keiichi. / A novel β-defensin structure : A potential strategy of big defensin for overcoming resistance by gram-positive bacteria. :: Biochemistry. 2008 ; 巻 47, 番号 40. pp. 10611-10619.
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