A novel ALL-L3 cell line, BALM-25, expressing both immunoglobulin light chains

Yoshinobu Matsuo, Hans G. Drexler, Akira Harashima, Katsuto Takenaka, Toshimichi Ishihara, Fumihiko Ishimaru, Kunzo Orita

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

A human acute lymphoblastic leukemia (ALL)-derived cell line, BALM-25, was established from the bone marrow specimen of a 59-year-old male patient with B-cell ALL L3 type (ALL-L3) at diagnosis. Immunophenotyping indicated mature B-cell characteristics including expression of cell surface and cytoplasmic immunoglobulin (Ig) chains, CD10, CD19, CD20, CD38, CD39, CD40, CD71, NU-B1 and HLA class II. T-cell and myeloid associated antigens tested were negative except CD5. BALM-25 cells have a morphological appearance typical for L3-type lymphoblasts. Regarding the expression of Ig chains, while the original leukemia cells expressed Ig λδμ and hence a single light (L) chain isotype, the established line revealed double L chain expression both at the cell surface and the cytoplasmic level. Definitive double L chain expression was confirmed by flow cytometry and Western blot analysis. Southern blot analysis demonstrated rearrangement of the IgJH, the Cκ and the Cλ genes. Cytogenetic analysis of BALM-25 revealed the following numerical and structural abnormalities: 55, X, add(X)(q12), + 2, add(3)(p21), + 5, add(7)(p13), add(11)(p11.2), add(11 (q?23), add(12)(p11.2), add(14)(q22), - 15, + 16, + 16,add(18)(11.2), + 20, + mar1, + mar2, + mar3, + mar,inc. The established cell line, BALM-25, provides an unlimited supply of cell material for analyzing the unique (patho)physiology of Ig expression in general and for clarifying the pathogenesis of this type of B-cell malignancy in particular.

本文言語英語
ページ(範囲)575-581
ページ数7
ジャーナルLeukemia and Lymphoma
45
3
DOI
出版ステータス出版済み - 3 2004

All Science Journal Classification (ASJC) codes

  • 血液学
  • 腫瘍学
  • 癌研究

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