TY - JOUR
T1 - A novel archaeal lineage in boiling hot springs around oyasukyo gorge (Akita, Japan)
AU - Asamatsu, Katsuhiro
AU - Yoshitake, Kai
AU - Saito, Makoto
AU - Prasitwuttisak, Wipoo
AU - Ishibashi, Junichiro
AU - Tsutsumi, Akihi
AU - Mustapha, Nurul Asyifah
AU - Maeda, Toshinari
AU - Yanagawa, Katsunori
N1 - Funding Information:
We thank Yukiko Yamasaki of the Yuzawa Geopark Promotion Council and the owner of Abe Ryokan for their support in the organization of field studies around Oyasukyo Gorge. This work was supported in part by a research grant from Yuzawa Geo-park and the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 20K05404.
Publisher Copyright:
© 2021, Japanese Society of Microbial Ecology. All rights reserved.
PY - 2021
Y1 - 2021
N2 - A novel deep-branching archaeal lineage was discovered at high-temperature hot springs around Oyasukyo Gorge in Akita Prefecture, Japan. Actively boiling hot spring water contained >1×104 microbes mL–1. The microbial community composition assessed by analyzing 16S rRNA gene amplicons revealed that the dominant bacterial phyla were Proteobacteria and Aquificae (>50% of the microbial composition) in samples collected in 2016 and 2019, respectively. Approximately 10% of the reads obtained in both years were not assigned to any taxonomy. The more detailed phylogenetic positions of the unassigned sequences identified using a clone library and phylogenetic tree showed that they formed a clade that was independent, distantly related to known phyla, and had low similarity (<82%) to all other sequences in available databases. The present results suggest that this novel archaeal phylum-level lineage thrives in boiling hot springs in Japan.
AB - A novel deep-branching archaeal lineage was discovered at high-temperature hot springs around Oyasukyo Gorge in Akita Prefecture, Japan. Actively boiling hot spring water contained >1×104 microbes mL–1. The microbial community composition assessed by analyzing 16S rRNA gene amplicons revealed that the dominant bacterial phyla were Proteobacteria and Aquificae (>50% of the microbial composition) in samples collected in 2016 and 2019, respectively. Approximately 10% of the reads obtained in both years were not assigned to any taxonomy. The more detailed phylogenetic positions of the unassigned sequences identified using a clone library and phylogenetic tree showed that they formed a clade that was independent, distantly related to known phyla, and had low similarity (<82%) to all other sequences in available databases. The present results suggest that this novel archaeal phylum-level lineage thrives in boiling hot springs in Japan.
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U2 - 10.1264/jsme2.ME21048
DO - 10.1264/jsme2.ME21048
M3 - Article
C2 - 34819404
AN - SCOPUS:85120179311
SN - 1342-6311
VL - 36
JO - Microbes and Environments
JF - Microbes and Environments
IS - 4
M1 - ME21048
ER -