A Novel Cell Fusion Method for Direct Cytoplasmic Transfer Using a Microfluidic Device

Ken Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda

研究成果: Chapter in Book/Report/Conference proceedingChapter

1 被引用数 (Scopus)

抄録

This chapter describes a novel cell fusion method for direct cytoplasmic transfer between live single cells. In order to realize this, we developed a microfluidic device which can make single cells pair at the position of microslit. Cell fusion was induced between the paired cells through a microslit by Sendai virus envelope (HVJ-E) to make strictured cytoplasmic connection. In the fused cell pairs, the nuclei in spread shape cells did not pass through the microslit. On the other hand, the nuclei in round shape cells migrated to the fusion partner leading to nuclear mixing. Supplementation with Y-27632, a ROCK inhibitor, prevented nuclear mixing and enhanced cell fusion rate. Mitochondria were transferred through the microslit under a condition of with or without Y-27632 supplementation. These data suggest that our cell fusion method using a newly developed microfluidic device can perform direct cytoplasmic transfer between live single cells.

本文言語英語
ホスト出版物のタイトルACS Symposium Series
編集者Yoshihiro Ito, Xuesi Chen, Inn-Kyu Kang
出版社American Chemical Society
ページ227-236
ページ数10
DOI
出版ステータス出版済み - 2017
外部発表はい

出版物シリーズ

名前ACS Symposium Series
1253
ISSN(印刷版)0097-6156
ISSN(電子版)1947-5918

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 化学工学(全般)

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