A novel crude oil emulsifier excreted in the culture supernatant of a marine bacterium, Myroides sp. strain SM1

Suppasil Maneerat, Takeshi Bamba, Kazuo Harada, Akio Kobayashi, Hidenori Yamada, Fusako Kawai

研究成果: Contribution to journalArticle査読

45 被引用数 (Scopus)

抄録

A marine bacterium, Myroides sp. SM1, can grow on weathered crude oil and show emulsification of it. The biosurfactant able to emulsify crude oil was excreted in culture supernatant of Myroides sp. SM1 grown on marine broth, which was extracted with chloroform/ methanol (1:1) at pH 7 and purified by normal and reverse phase silica gel column chromatographies. The compound was ninhydrin-positive, and the chemical structure was elucidated by nuclear magnetic resonance (NMR), infrared spectroscopy (IR), fast atom bombardment mass spectrometry, and gas chromatography-mass spectrometry (GCMS) to be a mixture of L-ornithine lipids, which were composed of L-ornithine and a different couple of iso-3-hydroxyfatty acid (C15-C17) and iso-fatty acid (C15 or C16) in a ratio of 1:1:1. The critical micelle concentration for a mixture of ornithine lipids was measured to be approximately 40 mg/1. A mixture of ornithine lipids exhibited emulsifying activity for crude oil in a broad range of pH, temperature, and salinity and showed higher surface activity for oil displacement test than other several artificial surfactants and a biosurfactant, surfactin.

本文言語英語
ページ(範囲)254-259
ページ数6
ジャーナルApplied Microbiology and Biotechnology
70
2
DOI
出版ステータス出版済み - 3 2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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