TY - JOUR
T1 - A novel ether-linked phytol-containing digalactosylglycerolipid in the marine green alga, Ulva pertusa
AU - Ishibashi, Yohei
AU - Nagamatsu, Yusuke
AU - Miyamoto, Tomofumi
AU - Matsunaga, Naoyuki
AU - Okino, Nozomu
AU - Yamaguchi, Kuniko
AU - Ito, Makoto
N1 - Funding Information:
This work was supported in part by Basic Research B from the Japanese Ministry of Education, Culture, Sports, Science and Technology (to M.I.), and the Japan Society for the Promotion of Science for Young Scientists (to Y.I.). We thank Drs. Hiroyuki Wariishi and Shigeo Kawaguchi, Kyushu University for their technical assistances. We are deeply grateful to Drs. Mari Inoue, Takashi Yuasa, Yohei Shimasaki, Tomonao Matsushita, and Eiji Goto, Kyushu University, and Dr. Shusei Sato and Ms. Reiko Muneto, the Kazusa DNA Research Institute, for donating photosynthetic organisms.
PY - 2014/10/3
Y1 - 2014/10/3
N2 - Galactosylglycerolipids (GGLs) and chlorophyll are characteristic components of chloroplast in photosynthetic organisms. Although chlorophyll is anchored to the thylakoid membrane by phytol (tetramethylhexadecenol), this isoprenoid alcohol has never been found as a constituent of GGLs. We here described a novel GGL, in which phytol was linked to the glycerol backbone via an ether linkage. This unique GGL was identified as an Alkaline-resistant and Endogalactosylceramidase (EGALC)-sensitive GlycoLipid (AEGL) in the marine green alga, Ulva pertusa. EGALC is an enzyme that is specific to the R-Galα/β1-6Galβ1-structure of galactolipids. The structure of U. pertusa AEGL was determined following its purification to 1-O-phytyl-3-O-Galα1-6Galβ1-sn-glycerol by mass spectrometric and nuclear magnetic resonance analyses. AEGLs were ubiquitously distributed in not only green, but also red and brown marine algae; however, they were rarely detected in terrestrial plants, eukaryotic phytoplankton, or cyanobacteria.
AB - Galactosylglycerolipids (GGLs) and chlorophyll are characteristic components of chloroplast in photosynthetic organisms. Although chlorophyll is anchored to the thylakoid membrane by phytol (tetramethylhexadecenol), this isoprenoid alcohol has never been found as a constituent of GGLs. We here described a novel GGL, in which phytol was linked to the glycerol backbone via an ether linkage. This unique GGL was identified as an Alkaline-resistant and Endogalactosylceramidase (EGALC)-sensitive GlycoLipid (AEGL) in the marine green alga, Ulva pertusa. EGALC is an enzyme that is specific to the R-Galα/β1-6Galβ1-structure of galactolipids. The structure of U. pertusa AEGL was determined following its purification to 1-O-phytyl-3-O-Galα1-6Galβ1-sn-glycerol by mass spectrometric and nuclear magnetic resonance analyses. AEGLs were ubiquitously distributed in not only green, but also red and brown marine algae; however, they were rarely detected in terrestrial plants, eukaryotic phytoplankton, or cyanobacteria.
UR - http://www.scopus.com/inward/record.url?scp=84907906460&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907906460&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2014.08.056
DO - 10.1016/j.bbrc.2014.08.056
M3 - Article
C2 - 25157808
AN - SCOPUS:84907906460
SN - 0006-291X
VL - 452
SP - 873
EP - 880
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
