A novel ether-linked phytol-containing digalactosylglycerolipid in the marine green alga, Ulva pertusa

Yohei Ishibashi, Yusuke Nagamatsu, Tomofumi Miyamoto, Naoyuki Matsunaga, Nozomu Okino, Kuniko Yamaguchi, Makoto Ito

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Galactosylglycerolipids (GGLs) and chlorophyll are characteristic components of chloroplast in photosynthetic organisms. Although chlorophyll is anchored to the thylakoid membrane by phytol (tetramethylhexadecenol), this isoprenoid alcohol has never been found as a constituent of GGLs. We here described a novel GGL, in which phytol was linked to the glycerol backbone via an ether linkage. This unique GGL was identified as an Alkaline-resistant and Endogalactosylceramidase (EGALC)-sensitive GlycoLipid (AEGL) in the marine green alga, Ulva pertusa. EGALC is an enzyme that is specific to the R-Galα/β1-6Galβ1-structure of galactolipids. The structure of U. pertusa AEGL was determined following its purification to 1-O-phytyl-3-O-Galα1-6Galβ1-sn-glycerol by mass spectrometric and nuclear magnetic resonance analyses. AEGLs were ubiquitously distributed in not only green, but also red and brown marine algae; however, they were rarely detected in terrestrial plants, eukaryotic phytoplankton, or cyanobacteria.

元の言語英語
ページ(範囲)873-880
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
452
発行部数4
DOI
出版物ステータス出版済み - 10 3 2014

Fingerprint

Ulva
Phytol
Chlorophyta
Chlorophyll
Algae
Ether
Glycerol
Galactolipids
Phaeophyta
Phytoplankton
Thylakoids
Glycolipids
Terpenes
Cyanobacteria
Chloroplasts
Purification
Magnetic Resonance Spectroscopy
Alcohols
Nuclear magnetic resonance
Membranes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

A novel ether-linked phytol-containing digalactosylglycerolipid in the marine green alga, Ulva pertusa. / Ishibashi, Yohei; Nagamatsu, Yusuke; Miyamoto, Tomofumi; Matsunaga, Naoyuki; Okino, Nozomu; Yamaguchi, Kuniko; Ito, Makoto.

:: Biochemical and Biophysical Research Communications, 巻 452, 番号 4, 03.10.2014, p. 873-880.

研究成果: ジャーナルへの寄稿記事

@article{eb84facd76a641e4a71e3c70b398a60e,
title = "A novel ether-linked phytol-containing digalactosylglycerolipid in the marine green alga, Ulva pertusa",
abstract = "Galactosylglycerolipids (GGLs) and chlorophyll are characteristic components of chloroplast in photosynthetic organisms. Although chlorophyll is anchored to the thylakoid membrane by phytol (tetramethylhexadecenol), this isoprenoid alcohol has never been found as a constituent of GGLs. We here described a novel GGL, in which phytol was linked to the glycerol backbone via an ether linkage. This unique GGL was identified as an Alkaline-resistant and Endogalactosylceramidase (EGALC)-sensitive GlycoLipid (AEGL) in the marine green alga, Ulva pertusa. EGALC is an enzyme that is specific to the R-Galα/β1-6Galβ1-structure of galactolipids. The structure of U. pertusa AEGL was determined following its purification to 1-O-phytyl-3-O-Galα1-6Galβ1-sn-glycerol by mass spectrometric and nuclear magnetic resonance analyses. AEGLs were ubiquitously distributed in not only green, but also red and brown marine algae; however, they were rarely detected in terrestrial plants, eukaryotic phytoplankton, or cyanobacteria.",
author = "Yohei Ishibashi and Yusuke Nagamatsu and Tomofumi Miyamoto and Naoyuki Matsunaga and Nozomu Okino and Kuniko Yamaguchi and Makoto Ito",
year = "2014",
month = "10",
day = "3",
doi = "10.1016/j.bbrc.2014.08.056",
language = "English",
volume = "452",
pages = "873--880",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - A novel ether-linked phytol-containing digalactosylglycerolipid in the marine green alga, Ulva pertusa

AU - Ishibashi, Yohei

AU - Nagamatsu, Yusuke

AU - Miyamoto, Tomofumi

AU - Matsunaga, Naoyuki

AU - Okino, Nozomu

AU - Yamaguchi, Kuniko

AU - Ito, Makoto

PY - 2014/10/3

Y1 - 2014/10/3

N2 - Galactosylglycerolipids (GGLs) and chlorophyll are characteristic components of chloroplast in photosynthetic organisms. Although chlorophyll is anchored to the thylakoid membrane by phytol (tetramethylhexadecenol), this isoprenoid alcohol has never been found as a constituent of GGLs. We here described a novel GGL, in which phytol was linked to the glycerol backbone via an ether linkage. This unique GGL was identified as an Alkaline-resistant and Endogalactosylceramidase (EGALC)-sensitive GlycoLipid (AEGL) in the marine green alga, Ulva pertusa. EGALC is an enzyme that is specific to the R-Galα/β1-6Galβ1-structure of galactolipids. The structure of U. pertusa AEGL was determined following its purification to 1-O-phytyl-3-O-Galα1-6Galβ1-sn-glycerol by mass spectrometric and nuclear magnetic resonance analyses. AEGLs were ubiquitously distributed in not only green, but also red and brown marine algae; however, they were rarely detected in terrestrial plants, eukaryotic phytoplankton, or cyanobacteria.

AB - Galactosylglycerolipids (GGLs) and chlorophyll are characteristic components of chloroplast in photosynthetic organisms. Although chlorophyll is anchored to the thylakoid membrane by phytol (tetramethylhexadecenol), this isoprenoid alcohol has never been found as a constituent of GGLs. We here described a novel GGL, in which phytol was linked to the glycerol backbone via an ether linkage. This unique GGL was identified as an Alkaline-resistant and Endogalactosylceramidase (EGALC)-sensitive GlycoLipid (AEGL) in the marine green alga, Ulva pertusa. EGALC is an enzyme that is specific to the R-Galα/β1-6Galβ1-structure of galactolipids. The structure of U. pertusa AEGL was determined following its purification to 1-O-phytyl-3-O-Galα1-6Galβ1-sn-glycerol by mass spectrometric and nuclear magnetic resonance analyses. AEGLs were ubiquitously distributed in not only green, but also red and brown marine algae; however, they were rarely detected in terrestrial plants, eukaryotic phytoplankton, or cyanobacteria.

UR - http://www.scopus.com/inward/record.url?scp=84907906460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907906460&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2014.08.056

DO - 10.1016/j.bbrc.2014.08.056

M3 - Article

C2 - 25157808

AN - SCOPUS:84907906460

VL - 452

SP - 873

EP - 880

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -