A novel filtration method for cord blood processing using a polyester fabric filter

T. Shima, N. Forraz, N. Sato, T. Yamauchi, H. Iwasaki, K. Takenaka, K. Akashi, C. Mcguckin, T. Teshima

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

Introduction: Cord blood (CB) is being increasingly used as a source of hematopoietic stem cells for transplantation to treat diseases of the blood and immune systems, and there is an urgent need to expand CB banking worldwide. CB processing requires costly machinery or a clean room that hampers wider application of CBT particularly in the developing countries. Methods: We developed a novel filtration system using a nonchemical-coated and nonwoven polyester fabric filter, which traps cells through affinity and does not require centrifugation or potentially toxic chemicals. Results: Cell processing with the device resulted in minimum cell loss of total cells and CD34+ cells, without impairing the ability of CD34+ cells to engraft and differentiate both in vivo and in vitro. Conclusion: CB processing with this device is simple, cost-effective, and nontoxic without requiring costly equipment will thus facilitate international CB banking, which helps in meeting the increasing worldwide demand for CB for allogeneic hematopoietic stem cell transplantation.

本文言語英語
ページ(範囲)436-446
ページ数11
ジャーナルInternational Journal of Laboratory Hematology
35
4
DOI
出版ステータス出版済み - 8 2013

All Science Journal Classification (ASJC) codes

  • Hematology
  • Clinical Biochemistry
  • Biochemistry, medical

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