A novel fucosyl glycosphingolipid of brine shrimp that is highly sensitive to endoglycoceramidase

Xu Xu, Yasuhiro Horibata, Masanori Inagaki, Yoichiro Hama, Keishi Sakaguchi, Hatsumi M. Goda, Nozomu Okino, Makoto Ito

研究成果: ジャーナルへの寄稿学術誌査読

5 被引用数 (Scopus)

抄録

Endoglycoceramidase (EGCase; EC 3.2.1.123) is a glycohydrolase that hydrolyzes the glycosidic linkage between the oligosaccharide and ceramide of various glycosphingolipids. We previously reported that hydra produced EGCase to digest glycosphingolipids of brine shrimp (Artemia salina), a type of aquatic crustacean used as a diet for the culture of hydra (Horibata Y, Sakaguchi K, Okino N, Iida H, Inagaki M, Fujisawa T, Hama Y, Ito M. 2004. J Biol Chem. 279:33379-33389). We report here that a major glycosphingolipid of brine shrimp is unique in structure and highly sensitive to EGCase. The glycosphingolipid was extracted from freshly hatched brine shrimp by Folch's partition, followed by mild alkaline hydrolysis and purification with a Sep-Pak plus silica cartridge. The structure of brine shrimp glycosphingolipid was determined by gas chromatography, gas chromatography-mass spectrometry, fast-atom bombardment mass spectrometry, and 1 >H-NMR spectrometry to be GlcNAcα1-2Fucα1-3Manβ1-4Glcβ1- 1′Cer. Two major molecular species of the glycosphingolipid were identified; the sugar and sphingoid base of each were the same but the major fatty acid was C22:0 and 2-hydroxy C22:0, respectively. This is the first report describing the glycosphingolipid that has an internal fucosyl residue substituted with α1-2 N-acetylglucosaminyl residue. This study also suggests the biological relevance of the glycosphingolipid as a dietary source of hydra which possesses EGCase as a digestion enzyme.

本文言語英語
ページ(範囲)1446-1451
ページ数6
ジャーナルGlycobiology
19
12
DOI
出版ステータス出版済み - 2009

!!!All Science Journal Classification (ASJC) codes

  • 生化学

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