A novel hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonist prevents ischemic neuronal death

Eizo Marutani, Shizuko Kosugi, Kentaro Tokuda, Ashok Khatri, Rebecca Nguyen, Dmitriy N. Atochin, Kotaro Kida, Klaus Van Leyen, Ken Arai, Fumito Ichinose

研究成果: ジャーナルへの寄稿記事

47 引用 (Scopus)

抄録

Physiological levels of H2S exert neuroprotective effects, whereas high concentrations of H2S may cause neurotoxicity in part via activation ofNMDAR.To characterize the neuroprotective effects of combination of exogenous H2S and NMDAR antagonism, we synthesized a novel H2S-releasing NMDAR antagonist N-((1r,3R,5S,7r)-3,5- dimethyladamantan-1-yl)-4-(3-thioxo-3H-1,2-dithiol-4-yl)-benzamide (S-memantine) and examined its effects in vitro and in vivo. S-memantine was synthesized by chemically combining a slow releasing H2S donor 4-(3-thioxo-3H-1,2- dithiol-4-yl)-benzoic acid (ACS48) with a NMDAR antagonist memantine. S-memantine increased intracellular sulfide levels in human neuroblastoma cells (SH-SY5Y) 10-fold as high as that was achieved by ACS48. Incubation with S-memantine after reoxygenation following oxygen and glucose deprivation (OGD) protected SH-SY5Y cells and murine primary cortical neurons more markedly than did ACS48 or memantine. Glutamate-induced intracellular calcium accumulation in primary cortical neurons were aggravated by sodium sulfide (Na2S) or ACS48, but suppressed by memantine and S-memantine. S-memantine prevented glutamate-induced glutathione depletion in SH-SY5Y cells more markedly than did Na2S or ACS48. Administration of S-memantine after global cerebral ischemia and reperfusion more robustly decreased cerebral infarct volume and improved survival and neurological function of mice than did ACS48 or memantine. These results suggest that an H2S-releasing NMDAR antagonist derivative S-memantine prevents ischemic neuronal death, providing a novel therapeutic strategy for ischemic brain injury.

元の言語英語
ページ(範囲)32124-32135
ページ数12
ジャーナルJournal of Biological Chemistry
287
発行部数38
DOI
出版物ステータス出版済み - 9 14 2012

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Memantine
Hydrogen Sulfide
N-Methyl-D-Aspartate Receptors
Neuroprotective Agents
Neurons
Glutamic Acid
Benzoic Acid
Sulfides
Brain Ischemia
Neuroblastoma
Brain Injuries
Reperfusion
Glutathione
Brain

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

これを引用

A novel hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonist prevents ischemic neuronal death. / Marutani, Eizo; Kosugi, Shizuko; Tokuda, Kentaro; Khatri, Ashok; Nguyen, Rebecca; Atochin, Dmitriy N.; Kida, Kotaro; Van Leyen, Klaus; Arai, Ken; Ichinose, Fumito.

:: Journal of Biological Chemistry, 巻 287, 番号 38, 14.09.2012, p. 32124-32135.

研究成果: ジャーナルへの寄稿記事

Marutani, E, Kosugi, S, Tokuda, K, Khatri, A, Nguyen, R, Atochin, DN, Kida, K, Van Leyen, K, Arai, K & Ichinose, F 2012, 'A novel hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonist prevents ischemic neuronal death', Journal of Biological Chemistry, 巻. 287, 番号 38, pp. 32124-32135. https://doi.org/10.1074/jbc.M112.374124
Marutani, Eizo ; Kosugi, Shizuko ; Tokuda, Kentaro ; Khatri, Ashok ; Nguyen, Rebecca ; Atochin, Dmitriy N. ; Kida, Kotaro ; Van Leyen, Klaus ; Arai, Ken ; Ichinose, Fumito. / A novel hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonist prevents ischemic neuronal death. :: Journal of Biological Chemistry. 2012 ; 巻 287, 番号 38. pp. 32124-32135.
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abstract = "Physiological levels of H2S exert neuroprotective effects, whereas high concentrations of H2S may cause neurotoxicity in part via activation ofNMDAR.To characterize the neuroprotective effects of combination of exogenous H2S and NMDAR antagonism, we synthesized a novel H2S-releasing NMDAR antagonist N-((1r,3R,5S,7r)-3,5- dimethyladamantan-1-yl)-4-(3-thioxo-3H-1,2-dithiol-4-yl)-benzamide (S-memantine) and examined its effects in vitro and in vivo. S-memantine was synthesized by chemically combining a slow releasing H2S donor 4-(3-thioxo-3H-1,2- dithiol-4-yl)-benzoic acid (ACS48) with a NMDAR antagonist memantine. S-memantine increased intracellular sulfide levels in human neuroblastoma cells (SH-SY5Y) 10-fold as high as that was achieved by ACS48. Incubation with S-memantine after reoxygenation following oxygen and glucose deprivation (OGD) protected SH-SY5Y cells and murine primary cortical neurons more markedly than did ACS48 or memantine. Glutamate-induced intracellular calcium accumulation in primary cortical neurons were aggravated by sodium sulfide (Na2S) or ACS48, but suppressed by memantine and S-memantine. S-memantine prevented glutamate-induced glutathione depletion in SH-SY5Y cells more markedly than did Na2S or ACS48. Administration of S-memantine after global cerebral ischemia and reperfusion more robustly decreased cerebral infarct volume and improved survival and neurological function of mice than did ACS48 or memantine. These results suggest that an H2S-releasing NMDAR antagonist derivative S-memantine prevents ischemic neuronal death, providing a novel therapeutic strategy for ischemic brain injury.",
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AU - Khatri, Ashok

AU - Nguyen, Rebecca

AU - Atochin, Dmitriy N.

AU - Kida, Kotaro

AU - Van Leyen, Klaus

AU - Arai, Ken

AU - Ichinose, Fumito

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