A novel insertion pathway of mitochondrial outer membrane proteins with multiple transmembrane segments

Hidenori Otera, Yohsuke Taira, Chika Horie, Yurina Suzuki, Hiroyuki Suzuki, Kiyoko Setoguchi, Hiroki Kato, Toshihiko Oka, Katsuyoshi Mihara

研究成果: ジャーナルへの寄稿記事

69 引用 (Scopus)

抄録

The central channel Tom40 of the preprotein translocase of outer membrane (TOM) complex is thought to be responsible for the import of virtually all preproteins synthesized outside the mitochondria. In this study, we analyze the topogenesis of the peripheral benzodiazepine receptor (PBR), which integrates into the mitochondrial outer membrane (MOM) through five hydrophobic transmembrane segments (TMSs) and functions in cholesterol import into the inner membrane. Analyses of in vitro and in vivo import into TOM component-depleted mitochondria reveal that PBR import (1) depends on the import receptor Tom70 but requires neither the Tom20 and Tom22 import receptors nor the import channel Tom40, (2) shares the post-Tom70 pathway with the C-tail-anchored proteins, and (3) requires factors of the mitochondrial intermembrane space. Furthermore, membrane integration of mitofusins and mitochondrial ubiquitin ligase, the MOM proteins with two and four TMSs, respectively, proceeds through the same initial pathway. These findings reveal a previously unidentified pathway of the membrane integration of MOM proteins with multiple TMSs.

元の言語英語
ページ(範囲)1355-1363
ページ数9
ジャーナルJournal of Cell Biology
179
発行部数7
DOI
出版物ステータス出版済み - 12 31 2007

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Membrane Proteins
Membranes
Mitochondrial Membranes
GABA-A Receptors
Mitochondria
Ligases
Ubiquitin
Cholesterol
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

Otera, H., Taira, Y., Horie, C., Suzuki, Y., Suzuki, H., Setoguchi, K., ... Mihara, K. (2007). A novel insertion pathway of mitochondrial outer membrane proteins with multiple transmembrane segments. Journal of Cell Biology, 179(7), 1355-1363. https://doi.org/10.1083/jcb.200702143

A novel insertion pathway of mitochondrial outer membrane proteins with multiple transmembrane segments. / Otera, Hidenori; Taira, Yohsuke; Horie, Chika; Suzuki, Yurina; Suzuki, Hiroyuki; Setoguchi, Kiyoko; Kato, Hiroki; Oka, Toshihiko; Mihara, Katsuyoshi.

:: Journal of Cell Biology, 巻 179, 番号 7, 31.12.2007, p. 1355-1363.

研究成果: ジャーナルへの寄稿記事

Otera, H, Taira, Y, Horie, C, Suzuki, Y, Suzuki, H, Setoguchi, K, Kato, H, Oka, T & Mihara, K 2007, 'A novel insertion pathway of mitochondrial outer membrane proteins with multiple transmembrane segments', Journal of Cell Biology, 巻. 179, 番号 7, pp. 1355-1363. https://doi.org/10.1083/jcb.200702143
Otera, Hidenori ; Taira, Yohsuke ; Horie, Chika ; Suzuki, Yurina ; Suzuki, Hiroyuki ; Setoguchi, Kiyoko ; Kato, Hiroki ; Oka, Toshihiko ; Mihara, Katsuyoshi. / A novel insertion pathway of mitochondrial outer membrane proteins with multiple transmembrane segments. :: Journal of Cell Biology. 2007 ; 巻 179, 番号 7. pp. 1355-1363.
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AU - Setoguchi, Kiyoko

AU - Kato, Hiroki

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