A novel intracellular membrane-bound calcium-independent phospholipase A2

Haruki Tanaka, Ryu Takeya, Hideki Sumimoto

研究成果: Contribution to journalArticle査読

76 被引用数 (Scopus)

抄録

We have cloned human cDNA encoding a novel protein of 782 amino acids that contains the lipase consensus sequence Gly-Xaa-Ser-Xaa-Gly and several stretches surrounding the motif, which are homologous to those of the catalytic domain of cytosolic calcium-independent phospholipase A2 (iPLA2). When expressed in COS-7 cells, the protein predominantly exists in the membrane fraction and exhibits a phospholipase A2 activity in a calcium-independent manner. The transcript of the membrane-bound iPLA2 gene is ubiquitously observed as a single band of approximately 3.3 kb on Northern blot, with the most abundant expression in the skeletal muscle and heart. By a search of the database, we have also identified its putative C. elegans homologue, which shows 47% identity with that of human in the iPLA2 catalytic region. Thus the novel type of iPLA2 is evolutionarily well conserved, suggestive of its biological significance. (C) 2000 Academic Press.

本文言語英語
ページ(範囲)320-326
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
272
2
DOI
出版ステータス出版済み - 6 7 2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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