A novel maT-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6

Yuichi Kawanishi, Reiko Takaishi, Miki Morimoto, Yutaka Banno, Si Kab Nho, Hideaki Maekawa, Yumiko Nakajima

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

A complex type marner-like element (MLE) was isolated by PCR with genomic DNA of Bombyx mandarina. The clones of this MLE, homologous to the 'Cecropia-ITR-MLE' in BmTNML locus that we have isolated previously from B. mori, were roughly classified into four groups according to the state of inserted elements: (1) Containing a retrotransposon (L1Bm) and another element, (2) containing L1Bm, alone, (3) containing another element alone and (4) without insertions. This inserted element was named BmamaT1, as it was homologous to the B. mori MLE called Bmmar6, which was previously reported to be similar to a maT-family member, Bmmar1. Many of the current Cecropia-ITR-MLE clones had, as the BmamaT1 insertion site, the sequence of TA/TATA, which may be a transposition target site. Moreover, the BmamaT1 elements that inserted into the Cecropia-ITR-MLE region were highly homogeneous, making one group when a phylogenetic tree was made together with BmamaT1 members isolated directly from the B. mandarina genome. Many of the inserted type BmamaT1 elements had complete ORF which: possessed the so-called DDD catalytic triad. All these findings were taken to indicate that a large fraction of the BmamaT1 elements are still highly active.

元の言語英語
ページ(範囲)45-52
ページ数8
ジャーナルJournal of Insect Biotechnology and Sericology
77
発行部数1
出版物ステータス出版済み - 2 1 2008

Fingerprint

Cecropia
Morus
Dichlorodiphenyldichloroethane
Retroelements
DNA Transposable Elements
Bombyx
Bombyx mori
transposons
DNA
Clone Cells
Genes
Insertional Mutagenesis
clones
Open Reading Frames
transposition (genetics)
retrotransposons
Genome
Polymerase Chain Reaction
open reading frames
genomics

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

これを引用

A novel maT-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6. / Kawanishi, Yuichi; Takaishi, Reiko; Morimoto, Miki; Banno, Yutaka; Nho, Si Kab; Maekawa, Hideaki; Nakajima, Yumiko.

:: Journal of Insect Biotechnology and Sericology, 巻 77, 番号 1, 01.02.2008, p. 45-52.

研究成果: ジャーナルへの寄稿記事

@article{4a398b8fb3694c0a81193774d26b0346,
title = "A novel maT-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6",
abstract = "A complex type marner-like element (MLE) was isolated by PCR with genomic DNA of Bombyx mandarina. The clones of this MLE, homologous to the 'Cecropia-ITR-MLE' in BmTNML locus that we have isolated previously from B. mori, were roughly classified into four groups according to the state of inserted elements: (1) Containing a retrotransposon (L1Bm) and another element, (2) containing L1Bm, alone, (3) containing another element alone and (4) without insertions. This inserted element was named BmamaT1, as it was homologous to the B. mori MLE called Bmmar6, which was previously reported to be similar to a maT-family member, Bmmar1. Many of the current Cecropia-ITR-MLE clones had, as the BmamaT1 insertion site, the sequence of TA/TATA, which may be a transposition target site. Moreover, the BmamaT1 elements that inserted into the Cecropia-ITR-MLE region were highly homogeneous, making one group when a phylogenetic tree was made together with BmamaT1 members isolated directly from the B. mandarina genome. Many of the inserted type BmamaT1 elements had complete ORF which: possessed the so-called DDD catalytic triad. All these findings were taken to indicate that a large fraction of the BmamaT1 elements are still highly active.",
author = "Yuichi Kawanishi and Reiko Takaishi and Miki Morimoto and Yutaka Banno and Nho, {Si Kab} and Hideaki Maekawa and Yumiko Nakajima",
year = "2008",
month = "2",
day = "1",
language = "English",
volume = "77",
pages = "45--52",
journal = "Journal of Insect Biotechnology and Sericology",
issn = "1346-8073",
publisher = "Japanese Society of Sericultural Sciences",
number = "1",

}

TY - JOUR

T1 - A novel maT-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6

AU - Kawanishi, Yuichi

AU - Takaishi, Reiko

AU - Morimoto, Miki

AU - Banno, Yutaka

AU - Nho, Si Kab

AU - Maekawa, Hideaki

AU - Nakajima, Yumiko

PY - 2008/2/1

Y1 - 2008/2/1

N2 - A complex type marner-like element (MLE) was isolated by PCR with genomic DNA of Bombyx mandarina. The clones of this MLE, homologous to the 'Cecropia-ITR-MLE' in BmTNML locus that we have isolated previously from B. mori, were roughly classified into four groups according to the state of inserted elements: (1) Containing a retrotransposon (L1Bm) and another element, (2) containing L1Bm, alone, (3) containing another element alone and (4) without insertions. This inserted element was named BmamaT1, as it was homologous to the B. mori MLE called Bmmar6, which was previously reported to be similar to a maT-family member, Bmmar1. Many of the current Cecropia-ITR-MLE clones had, as the BmamaT1 insertion site, the sequence of TA/TATA, which may be a transposition target site. Moreover, the BmamaT1 elements that inserted into the Cecropia-ITR-MLE region were highly homogeneous, making one group when a phylogenetic tree was made together with BmamaT1 members isolated directly from the B. mandarina genome. Many of the inserted type BmamaT1 elements had complete ORF which: possessed the so-called DDD catalytic triad. All these findings were taken to indicate that a large fraction of the BmamaT1 elements are still highly active.

AB - A complex type marner-like element (MLE) was isolated by PCR with genomic DNA of Bombyx mandarina. The clones of this MLE, homologous to the 'Cecropia-ITR-MLE' in BmTNML locus that we have isolated previously from B. mori, were roughly classified into four groups according to the state of inserted elements: (1) Containing a retrotransposon (L1Bm) and another element, (2) containing L1Bm, alone, (3) containing another element alone and (4) without insertions. This inserted element was named BmamaT1, as it was homologous to the B. mori MLE called Bmmar6, which was previously reported to be similar to a maT-family member, Bmmar1. Many of the current Cecropia-ITR-MLE clones had, as the BmamaT1 insertion site, the sequence of TA/TATA, which may be a transposition target site. Moreover, the BmamaT1 elements that inserted into the Cecropia-ITR-MLE region were highly homogeneous, making one group when a phylogenetic tree was made together with BmamaT1 members isolated directly from the B. mandarina genome. Many of the inserted type BmamaT1 elements had complete ORF which: possessed the so-called DDD catalytic triad. All these findings were taken to indicate that a large fraction of the BmamaT1 elements are still highly active.

UR - http://www.scopus.com/inward/record.url?scp=40949093661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40949093661&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:40949093661

VL - 77

SP - 45

EP - 52

JO - Journal of Insect Biotechnology and Sericology

JF - Journal of Insect Biotechnology and Sericology

SN - 1346-8073

IS - 1

ER -