A novel mechanism for glucose side-chain formation in rhamnose-glucose polysaccharide synthesis

Kazuhisa Ozaki, Yukie Shibata, Yoshihisa Yamashita, Yoshio Nakano, Hiromasa Tsuda, Toshihiko Koga

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

We have cloned two genes (rgpH and rgpI) that encode proteins for the formation of the glucose side-chains of the Streptococcus mutans rhamnose-glucose polysaccharide (RGP), which consists of a rhamnan backbone with glucose side-chains. The roles of rgpH and rgpI were evaluated in a rhamnan-synthesizing Escherichia coli. An E. coli strain that harbored rgpH reacted with antiserum directed against complete RGP, whereas the E. coli strain that carried rgpI did not react with this antiserum. Although E. coli:rgpH reacted strongly with rhamnan-specific antiserum, co-transformation of this strain with rgpI increased the number of glucose side-chains and decreased immunoreactivity with the rhamnan-specific antiserum significantly. These results suggest that two genes are involved in side-chain formation during S. mutans RGP synthesis in E. coli: one gene encodes a glucosyltransferase, and the other gene probably controls the frequency of branching. This is the first report to identify a gene that is involved in regulation of branching frequency in polysaccharide synthesis.

元の言語英語
ページ(範囲)159-163
ページ数5
ジャーナルFEBS Letters
532
発行部数1-2
DOI
出版物ステータス出版済み - 12 4 2002

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Rhamnose
Polysaccharides
Escherichia coli
Glucose
Genes
Immune Sera
Streptococcus mutans
Glucosyltransferases
rhamnan
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

A novel mechanism for glucose side-chain formation in rhamnose-glucose polysaccharide synthesis. / Ozaki, Kazuhisa; Shibata, Yukie; Yamashita, Yoshihisa; Nakano, Yoshio; Tsuda, Hiromasa; Koga, Toshihiko.

:: FEBS Letters, 巻 532, 番号 1-2, 04.12.2002, p. 159-163.

研究成果: ジャーナルへの寄稿記事

Ozaki, Kazuhisa ; Shibata, Yukie ; Yamashita, Yoshihisa ; Nakano, Yoshio ; Tsuda, Hiromasa ; Koga, Toshihiko. / A novel mechanism for glucose side-chain formation in rhamnose-glucose polysaccharide synthesis. :: FEBS Letters. 2002 ; 巻 532, 番号 1-2. pp. 159-163.
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AU - Tsuda, Hiromasa

AU - Koga, Toshihiko

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