A parallel affinity purification method for selective isolation of polyubiquitinated proteins

Kazuhisa Ota, Keiji Kito, Shun Ichiro Iemura, Tohru Natsume, Takashi Ito

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

We developed a parallel affinity purification (PAP) procedure, in which ubiquitinated proteins are purified from the cells that coexpress two affinity-tagged ubiquitins by sequential use of affinity chromatography specific to each tag. In contrast with previous procedures using a single affinity-tagged ubiquitin, the PAP eliminates highly abundant ubiquitin monomers and monoubiquitinated proteins to selectively enrich proteins bearing both affinity-tags, or poly- and multiubiquitinated proteins. Accordingly, it would serve as a powerful method to facilitate mass-spectrometric identification of ubiquitinated proteins.

元の言語英語
ページ(範囲)3004-3007
ページ数4
ジャーナルProteomics
8
発行部数15
DOI
出版物ステータス出版済み - 8 1 2008

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Ubiquitinated Proteins
Purification
Ubiquitin
Bearings (structural)
Ubiquitins
Affinity chromatography
Proteins
Affinity Chromatography
Monomers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

A parallel affinity purification method for selective isolation of polyubiquitinated proteins. / Ota, Kazuhisa; Kito, Keiji; Iemura, Shun Ichiro; Natsume, Tohru; Ito, Takashi.

:: Proteomics, 巻 8, 番号 15, 01.08.2008, p. 3004-3007.

研究成果: ジャーナルへの寄稿記事

Ota, Kazuhisa ; Kito, Keiji ; Iemura, Shun Ichiro ; Natsume, Tohru ; Ito, Takashi. / A parallel affinity purification method for selective isolation of polyubiquitinated proteins. :: Proteomics. 2008 ; 巻 8, 番号 15. pp. 3004-3007.
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AU - Ito, Takashi

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