A parallel affinity purification method for selective isolation of polyubiquitinated proteins

Kazuhisa Ota, Keiji Kito, Shun Ichiro Iemura, Tohru Natsume, Takashi Ito

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

We developed a parallel affinity purification (PAP) procedure, in which ubiquitinated proteins are purified from the cells that coexpress two affinity-tagged ubiquitins by sequential use of affinity chromatography specific to each tag. In contrast with previous procedures using a single affinity-tagged ubiquitin, the PAP eliminates highly abundant ubiquitin monomers and monoubiquitinated proteins to selectively enrich proteins bearing both affinity-tags, or poly- and multiubiquitinated proteins. Accordingly, it would serve as a powerful method to facilitate mass-spectrometric identification of ubiquitinated proteins.

本文言語英語
ページ(範囲)3004-3007
ページ数4
ジャーナルProteomics
8
15
DOI
出版ステータス出版済み - 8 1 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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