A peptide microarray fabricated on a non-fouling phosphatidylcholinepolymer-coated surface for a high-fidelity analysis of a cellular kinome

H. Ikeda, J. Kamimoto, T. Yamamoto, A. Hata, Y. Otsubo, T. Niidome, M. Fukushima, T. Mori, Y. Katayama

    研究成果: ジャーナルへの寄稿学術誌査読

    6 被引用数 (Scopus)

    抄録

    Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.

    本文言語英語
    ページ(範囲)4419-4425
    ページ数7
    ジャーナルCurrent Medicinal Chemistry
    20
    35
    DOI
    出版ステータス出版済み - 11月 2013

    !!!All Science Journal Classification (ASJC) codes

    • 生化学
    • 分子医療
    • 薬理学
    • 創薬
    • 有機化学

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