A peptide microarray fabricated on a non-fouling phosphatidylcholinepolymer-coated surface for a high-fidelity analysis of a cellular kinome

H. Ikeda, J. Kamimoto, T. Yamamoto, A. Hata, Y. Otsubo, T. Niidome, M. Fukushima, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.

元の言語英語
ページ(範囲)4419-4425
ページ数7
ジャーナルCurrent Medicinal Chemistry
20
発行部数35
DOI
出版物ステータス出版済み - 11 1 2013

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Microarrays
Peptides
Phosphatidylcholines
Polymers
Signal-To-Noise Ratio
Plastics
Glass
Signal to noise ratio
Fabrication

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

これを引用

A peptide microarray fabricated on a non-fouling phosphatidylcholinepolymer-coated surface for a high-fidelity analysis of a cellular kinome. / Ikeda, H.; Kamimoto, J.; Yamamoto, T.; Hata, A.; Otsubo, Y.; Niidome, T.; Fukushima, M.; Mori, Takeshi; Katayama, Yoshiki.

:: Current Medicinal Chemistry, 巻 20, 番号 35, 01.11.2013, p. 4419-4425.

研究成果: ジャーナルへの寄稿記事

Ikeda, H. ; Kamimoto, J. ; Yamamoto, T. ; Hata, A. ; Otsubo, Y. ; Niidome, T. ; Fukushima, M. ; Mori, Takeshi ; Katayama, Yoshiki. / A peptide microarray fabricated on a non-fouling phosphatidylcholinepolymer-coated surface for a high-fidelity analysis of a cellular kinome. :: Current Medicinal Chemistry. 2013 ; 巻 20, 番号 35. pp. 4419-4425.
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