TY - JOUR
T1 - A peptide microarray fabricated on a non-fouling phosphatidylcholinepolymer-coated surface for a high-fidelity analysis of a cellular kinome
AU - Ikeda, H.
AU - Kamimoto, J.
AU - Yamamoto, T.
AU - Hata, A.
AU - Otsubo, Y.
AU - Niidome, T.
AU - Fukushima, M.
AU - Mori, T.
AU - Katayama, Y.
PY - 2013/11
Y1 - 2013/11
N2 - Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.
AB - Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.
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U2 - 10.2174/09298673113209990142
DO - 10.2174/09298673113209990142
M3 - Article
C2 - 23746278
AN - SCOPUS:84887939826
VL - 20
SP - 4419
EP - 4425
JO - Current Medicinal Chemistry
JF - Current Medicinal Chemistry
SN - 0929-8673
IS - 35
ER -