抄録
Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 4419-4425 |
| ページ数 | 7 |
| ジャーナル | Current Medicinal Chemistry |
| 巻 | 20 |
| 発行部数 | 35 |
| DOI | |
| 出版物ステータス | 出版済み - 11 1 2013 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Medicine
- Pharmacology
- Drug Discovery
- Organic Chemistry
これを引用
A peptide microarray fabricated on a non-fouling phosphatidylcholinepolymer-coated surface for a high-fidelity analysis of a cellular kinome. / Ikeda, H.; Kamimoto, J.; Yamamoto, T.; Hata, A.; Otsubo, Y.; Niidome, T.; Fukushima, M.; Mori, Takeshi; Katayama, Yoshiki.
：: Current Medicinal Chemistry, 巻 20, 番号 35, 01.11.2013, p. 4419-4425.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - A peptide microarray fabricated on a non-fouling phosphatidylcholinepolymer-coated surface for a high-fidelity analysis of a cellular kinome
AU - Ikeda, H.
AU - Kamimoto, J.
AU - Yamamoto, T.
AU - Hata, A.
AU - Otsubo, Y.
AU - Niidome, T.
AU - Fukushima, M.
AU - Mori, Takeshi
AU - Katayama, Yoshiki
PY - 2013/11/1
Y1 - 2013/11/1
N2 - Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.
AB - Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.
UR - http://www.scopus.com/inward/record.url?scp=84887939826&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887939826&partnerID=8YFLogxK
U2 - 10.2174/09298673113209990142
DO - 10.2174/09298673113209990142
M3 - Article
C2 - 23746278
AN - SCOPUS:84887939826
VL - 20
SP - 4419
EP - 4425
JO - Current Medicinal Chemistry
JF - Current Medicinal Chemistry
SN - 0929-8673
IS - 35
ER -