A peptide sequence controls the physical properties of nanoparticles formed by peptide-polymer conjugates that respond to a protein kinase A signal

Tatsuhiko Sonoda, Takashi Nogami, Jun Oishi, Masaharu Murata, Takuro Niidome, Yoshiki Katayama

    研究成果: ジャーナルへの寄稿学術誌査読

    14 被引用数 (Scopus)

    抄録

    We previously reported that poly(N-isopropylacrylamide) grafted with Peptide 1 (-GLRRASLG) and poly(ethylene glycol) changed its physical properties in response to an intracellular protein phosphorylation signal, protein kinase A (PKA) (Katayama, Y. et al. (2001) Macromolecules 34, 905). In this study, we investigated the effect of changing peptide structure on the lower critical solution temperature (LCST) of peptide-polymer conjugates, before and after phosphorylation with PKA. For Peptide 2 (Ac-LRRASL-), which has a formal net charge of +2 at physiological pH, the LCST of the conjugate decreased on phosphorylation. In contrast, the LCSTs of the conjugates with Peptide 3 (-ALRRASLE) and Peptide 4 (Ac-DWDALRRASL-), which have neutral net charges, were greatly increased. This suggests that the LCST of the polymer was mainly governed by two factors: the change in hydration around the polymer chain and the interpeptide electrostatic repulsion, resulting from phosphorylation. These polymers have potential for use as drug capsules that respond to cellular conditions.

    本文言語英語
    ページ(範囲)1542-1546
    ページ数5
    ジャーナルBioconjugate Chemistry
    16
    6
    DOI
    出版ステータス出版済み - 11月 1 2005

    !!!All Science Journal Classification (ASJC) codes

    • 化学 (全般)
    • 有機化学
    • 臨床生化学
    • 生化学、遺伝学、分子生物学(全般)
    • 生化学

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