A peptide sequence controls the physical properties of nanoparticles formed by peptide-polymer conjugates that respond to a protein kinase A signal

Tatsuhiko Sonoda, Takashi Nogami, Jun Oishi, Masaharu Murata, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

We previously reported that poly(N-isopropylacrylamide) grafted with Peptide 1 (-GLRRASLG) and poly(ethylene glycol) changed its physical properties in response to an intracellular protein phosphorylation signal, protein kinase A (PKA) (Katayama, Y. et al. (2001) Macromolecules 34, 905). In this study, we investigated the effect of changing peptide structure on the lower critical solution temperature (LCST) of peptide-polymer conjugates, before and after phosphorylation with PKA. For Peptide 2 (Ac-LRRASL-), which has a formal net charge of +2 at physiological pH, the LCST of the conjugate decreased on phosphorylation. In contrast, the LCSTs of the conjugates with Peptide 3 (-ALRRASLE) and Peptide 4 (Ac-DWDALRRASL-), which have neutral net charges, were greatly increased. This suggests that the LCST of the polymer was mainly governed by two factors: the change in hydration around the polymer chain and the interpeptide electrostatic repulsion, resulting from phosphorylation. These polymers have potential for use as drug capsules that respond to cellular conditions.

元の言語英語
ページ(範囲)1542-1546
ページ数5
ジャーナルBioconjugate Chemistry
16
発行部数6
DOI
出版物ステータス出版済み - 11 1 2005

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Cyclic AMP-Dependent Protein Kinases
Nanoparticles
Peptides
Polymers
Phosphorylation
Physical properties
Proteins
Temperature
Ethylene Glycol
Static Electricity
Macromolecules
Hydration
Polyethylene glycols
Capsules
Electrostatics
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

これを引用

A peptide sequence controls the physical properties of nanoparticles formed by peptide-polymer conjugates that respond to a protein kinase A signal. / Sonoda, Tatsuhiko; Nogami, Takashi; Oishi, Jun; Murata, Masaharu; Niidome, Takuro; Katayama, Yoshiki.

:: Bioconjugate Chemistry, 巻 16, 番号 6, 01.11.2005, p. 1542-1546.

研究成果: ジャーナルへの寄稿記事

Sonoda, Tatsuhiko ; Nogami, Takashi ; Oishi, Jun ; Murata, Masaharu ; Niidome, Takuro ; Katayama, Yoshiki. / A peptide sequence controls the physical properties of nanoparticles formed by peptide-polymer conjugates that respond to a protein kinase A signal. :: Bioconjugate Chemistry. 2005 ; 巻 16, 番号 6. pp. 1542-1546.
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AU - Niidome, Takuro

AU - Katayama, Yoshiki

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