TY - JOUR
T1 - A phytochemical-sensing strategy based on mass spectrometry imaging and metabolic profiling for understanding the functionality of the medicinal herb green tea
AU - Fujimura, Yoshinori
AU - Miura, Daisuke
AU - Tachibana, Hirofumi
N1 - Funding Information:
Acknowledgments: This work was supported by MEXT Funding-Project for Developing Innovation Systems-Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program in Japan. This work was also supported in part by JSPS KAKENHI Grants Number 17H03819 (to Yoshinori Fujimura) and 26713020 (to Daisuke Miura).
Publisher Copyright:
© 2017 by the authors.
PY - 2017/10
Y1 - 2017/10
N2 - Low-molecular-weight phytochemicals have health benefits and reduce the risk of diseases, but the mechanisms underlying their activities have remained elusive because of the lack of a methodology that can easily visualize the exact behavior of such small molecules. Recently, we developed an in situ label-free imaging technique, called mass spectrometry imaging, for visualizing spatially-resolved biotransformations based on simultaneous mapping of the major bioactive green tea polyphenol and its phase II metabolites. In addition, we established a mass spectrometry-based metabolic profiling technique capable of evaluating the bioactivities of diverse green tea extracts, which contain multiple phytochemicals, by focusing on their compositional balances. This methodology allowed us to simultaneously evaluate the relative contributions of the multiple compounds present in a multicomponent system to its bioactivity. This review highlights small molecule-sensing techniques for visualizing the complex behaviors of herbal components and linking such information to an enhanced understanding of the functionalities of multicomponent medicinal herbs.
AB - Low-molecular-weight phytochemicals have health benefits and reduce the risk of diseases, but the mechanisms underlying their activities have remained elusive because of the lack of a methodology that can easily visualize the exact behavior of such small molecules. Recently, we developed an in situ label-free imaging technique, called mass spectrometry imaging, for visualizing spatially-resolved biotransformations based on simultaneous mapping of the major bioactive green tea polyphenol and its phase II metabolites. In addition, we established a mass spectrometry-based metabolic profiling technique capable of evaluating the bioactivities of diverse green tea extracts, which contain multiple phytochemicals, by focusing on their compositional balances. This methodology allowed us to simultaneously evaluate the relative contributions of the multiple compounds present in a multicomponent system to its bioactivity. This review highlights small molecule-sensing techniques for visualizing the complex behaviors of herbal components and linking such information to an enhanced understanding of the functionalities of multicomponent medicinal herbs.
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U2 - 10.3390/molecules22101621
DO - 10.3390/molecules22101621
M3 - Review article
C2 - 28953237
AN - SCOPUS:85032907658
VL - 22
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 10
M1 - 1621
ER -