A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion

Kazuo Sakurai, Seiji Shinkai

研究成果: Contribution to conferencePaper査読

抄録

A β-(1→3)-D-glucan schizophyllan (SPG) forms a stoichiometric complex with some polynucleotides. This paper describes our attempt to apply the SPG complex to deliver CpG DNA to endosome in order to enhance cytokine secretion. To increase cellular up-take, we introduced spermine, arginine-glycine-aspartic acid tripeptide, octaarginine, or cholesterol to the SPG side chain. The chemically modified SPG shows essentially no-cytotoxicity. When CpG DMA complex made therefrom was exposed to macrophages, dramatic enhancement in the cytokine secretion was observed. It increased 5-10 times from the naked dose, and 100 times from the background. This performance promises that SPG can be an excellent carrier for CpG DNA. The paper will include a recent result for in-vivo assay carried out by Profs. Akira and Ishii at Osaka Univ.

本文言語英語
ページ5110-5111
ページ数2
出版ステータス出版済み - 12 1 2005
イベント54th SPSJ Symposium on Macromolecules - Yamagata, 日本
継続期間: 9 20 20059 22 2005

その他

その他54th SPSJ Symposium on Macromolecules
国/地域日本
CityYamagata
Period9/20/059/22/05

All Science Journal Classification (ASJC) codes

  • 工学(全般)

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