A polysaccharide carrier to effectively deliver native phosphodiester CpG DNA to antigen-presenting cells

Naohiko Shimada, Cevayir Coban, Yoichi Takeda, Masami Mizu, Jusaku Minari, Takahisa Anada, Yuichi Torii, Seiji Shinkai, Shizuo Akira, Ken J. Ishii, Kazuo Sakurai

研究成果: Contribution to journalArticle査読

18 被引用数 (Scopus)

抄録

Oligodeoxynucleotides containing unmethylated CpG sequences (CpG DNAs) activate the vertebrate innate immune system via toll-like receptor 9 (TLR-9). Although CpG DNA is a promising immunotherapeutic agent, its short circulation time in biological fluids due to nuclease is the major drawback. This paper proposes that a natural polysaccharide called schizophyllan (SPG) can be used as an effective CpG DNA carrier because SPG can complex with CpG DNA and the resultant complex shows the nuclease resistance of the bound DNA. In order to increase cellular uptake in vitro, we chemically attached spermine, cholesterol, arginine octamer, or RGD peptide to SPG. The complexes made of the chemically modified SPG and CpG DNA having a phosphorothioate (PS) or phosphodiester (PO) backbone led to increased secretion of cytokines of about 4- to 15-fold, compared with the uncomplexed dose. Furthermore, when PO CpG DNA was complexed with unmodified SPG, the IL-12 level increased by almost 3- to 11-fold compared with the naked dose. The PO CpG DNA/unmodified SPG complex data suggested that unmodified SPG might effectively deliver PO in vivo due to the electrically neutral nature of unmodified SPG. When the complexed CpG DNAs were injected intraperitoneally, a large amount of IL-12 production was observed compared with the uncomplexed material. Both in vivo and vitro assays indicated that the SPG complex may be of use for CpG DNA therapy.

本文言語英語
ページ(範囲)1280-1286
ページ数7
ジャーナルBioconjugate Chemistry
18
4
DOI
出版ステータス出版済み - 7 2007

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体医工学
  • 薬理学
  • 薬科学
  • 有機化学

フィンガープリント

「A polysaccharide carrier to effectively deliver native phosphodiester CpG DNA to antigen-presenting cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル