A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

Shujiro Shiosaki, Takanobu Nobori, Takeshi Mori, Riki Toita, Yuta Nakamura, Chan Woo Kim, Tatsuhiro Yamamoto, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.

元の言語英語
ページ(範囲)5592-5594
ページ数3
ジャーナルChemical Communications
49
発行部数49
DOI
出版物ステータス出版済み - 6 21 2013

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Protein Kinases
Peptides
Semiconductor quantum dots
Assays
Proteins
Phosphorylation
Protein Kinase Inhibitors
Substrates

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

これを引用

A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides. / Shiosaki, Shujiro; Nobori, Takanobu; Mori, Takeshi; Toita, Riki; Nakamura, Yuta; Kim, Chan Woo; Yamamoto, Tatsuhiro; Niidome, Takuro; Katayama, Yoshiki.

:: Chemical Communications, 巻 49, 番号 49, 21.06.2013, p. 5592-5594.

研究成果: ジャーナルへの寄稿記事

Shiosaki, S, Nobori, T, Mori, T, Toita, R, Nakamura, Y, Kim, CW, Yamamoto, T, Niidome, T & Katayama, Y 2013, 'A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides', Chemical Communications, 巻. 49, 番号 49, pp. 5592-5594. https://doi.org/10.1039/c3cc41680a
Shiosaki, Shujiro ; Nobori, Takanobu ; Mori, Takeshi ; Toita, Riki ; Nakamura, Yuta ; Kim, Chan Woo ; Yamamoto, Tatsuhiro ; Niidome, Takuro ; Katayama, Yoshiki. / A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides. :: Chemical Communications. 2013 ; 巻 49, 番号 49. pp. 5592-5594.
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