A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS

Yuki Otsubo, Hiromu Ikeda, Junpei Kamimoto, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

    研究成果: ジャーナルへの寄稿学術誌査読

    抄録

    A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly compared utlizing the mass-unit difference of the subsrate peptides with different isotope labels. We successfully evaluated the effect of an inhibitory drug on the cellular protein kinase acitivity.

    本文言語英語
    ページ(範囲)658-660
    ページ数3
    ジャーナルChemistry Letters
    43
    5
    DOI
    出版ステータス出版済み - 2014

    !!!All Science Journal Classification (ASJC) codes

    • 化学 (全般)

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