A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS

Yuki Otsubo, Hiromu Ikeda, Junpei Kamimoto, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

抄録

A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly compared utlizing the mass-unit difference of the subsrate peptides with different isotope labels. We successfully evaluated the effect of an inhibitory drug on the cellular protein kinase acitivity.

元の言語英語
ページ(範囲)658-660
ページ数3
ジャーナルChemistry Letters
43
発行部数5
DOI
出版物ステータス出版済み - 1 1 2014

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Protein Kinases
Isotopes
Peptides
Labeling
Labels
Substrates
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

これを引用

A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS. / Otsubo, Yuki; Ikeda, Hiromu; Kamimoto, Junpei; Niidome, Takuro; Mori, Takeshi; Katayama, Yoshiki.

:: Chemistry Letters, 巻 43, 番号 5, 01.01.2014, p. 658-660.

研究成果: ジャーナルへの寄稿記事

Otsubo, Yuki ; Ikeda, Hiromu ; Kamimoto, Junpei ; Niidome, Takuro ; Mori, Takeshi ; Katayama, Yoshiki. / A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS. :: Chemistry Letters. 2014 ; 巻 43, 番号 5. pp. 658-660.
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