A rapid ATP-based method for determining active microbial populations in mineral leach liquors

Naoko Okibe, D. Barrie Johnson

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

An empirical technique for the rapid assessment of active biomass of acidophilic microorganisms in pure cultures and in leach liquors from mineral bio-processing operations is described. The method is based on the measurement of ATP concentrations in test solutions, using commercially-available kits and a portable luminometer. The ATP contents of five Gram-negative biomining bacteria mineral-degrading acidophiles assayed varied from 0.87 × 10-20 to 18 × 10-20 mol per cell in actively-growing cultures. Depletion of ferrous iron in cultures of a strain of Leptospirillum ferriphilum resulted in rapid mortality of the iron-oxidizing bacterium (denoted by decreasing numbers of plate isolates) and corresponding changes in ATP concentrations in culture aliquots. The technique was applied successfully to mineral leachates from stirred tank biomining operations, where it was shown to determine populations of active microorganisms in liquors where most of the cells present were not viable. The method is highly sensitive (detecting as few as 103 acidophiles ml-1 of test solution) and would therefore have generic application in monitoring microbial populations in other types of biomining operations, such as heap bioreactors.

元の言語英語
ページ(範囲)195-198
ページ数4
ジャーナルHydrometallurgy
108
発行部数3-4
DOI
出版物ステータス出版済み - 7 1 2011

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Adenosinetriphosphate
Minerals
Adenosine Triphosphate
Microorganisms
Bacteria
Iron
Bioreactors
Biomass
Monitoring
Processing

All Science Journal Classification (ASJC) codes

  • Industrial and Manufacturing Engineering
  • Metals and Alloys
  • Materials Chemistry

これを引用

A rapid ATP-based method for determining active microbial populations in mineral leach liquors. / Okibe, Naoko; Johnson, D. Barrie.

:: Hydrometallurgy, 巻 108, 番号 3-4, 01.07.2011, p. 195-198.

研究成果: ジャーナルへの寄稿記事

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