A rapid bacteria detection technique utilizing impedance measurement combined with positive and negative dielectrophoresis

R. Hamada, H. Takayama, Y. Shonishi, L. Mao, Michihiko Nakano, Junya Suehiro

研究成果: ジャーナルへの寄稿記事

42 引用 (Scopus)

抄録

In this study, a bacterial detection technique and device that utilizes advantages of both positive and negative dielectrophoresis (DEP) has been proposed and demonstrated. The device has two microelectrodes, which serve as a bacteria concentrator using negative DEP (n-DEP) and as a bacteria detector using positive DEP (p-DEP), respectively. Bacteria flowing into the device are repelled under action of n-DEP force exerted by the first microelectrode, and are pushed toward the second microelectrode situated at the downstream. Then concentrated bacteria are finally captured by p-DEP on the second microelectrode and detected by dielectrophoretic impedance measurement (DEPIM) method. The numerical simulations and experiments proved that n-DEP concentrator could make DEPIM sensitivity two times higher than that without n-DEP as a result of increased number of bacteria trapped on the p-DEP microelectrode.

元の言語英語
ページ(範囲)439-445
ページ数7
ジャーナルSensors and Actuators, B: Chemical
181
DOI
出版物ステータス出版済み - 3 25 2013

Fingerprint

Microelectrodes
impedance measurement
Electrophoresis
bacteria
Bacteria
concentrators
1-(2-(dodecyloxy)ethyl)pyrrolidine hydrochloride
Detectors
sensitivity
detectors
Computer simulation
simulation
Experiments

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

これを引用

A rapid bacteria detection technique utilizing impedance measurement combined with positive and negative dielectrophoresis. / Hamada, R.; Takayama, H.; Shonishi, Y.; Mao, L.; Nakano, Michihiko; Suehiro, Junya.

:: Sensors and Actuators, B: Chemical, 巻 181, 25.03.2013, p. 439-445.

研究成果: ジャーナルへの寄稿記事

@article{53eb19c15f774bb2998dcbac0970dec6,
title = "A rapid bacteria detection technique utilizing impedance measurement combined with positive and negative dielectrophoresis",
abstract = "In this study, a bacterial detection technique and device that utilizes advantages of both positive and negative dielectrophoresis (DEP) has been proposed and demonstrated. The device has two microelectrodes, which serve as a bacteria concentrator using negative DEP (n-DEP) and as a bacteria detector using positive DEP (p-DEP), respectively. Bacteria flowing into the device are repelled under action of n-DEP force exerted by the first microelectrode, and are pushed toward the second microelectrode situated at the downstream. Then concentrated bacteria are finally captured by p-DEP on the second microelectrode and detected by dielectrophoretic impedance measurement (DEPIM) method. The numerical simulations and experiments proved that n-DEP concentrator could make DEPIM sensitivity two times higher than that without n-DEP as a result of increased number of bacteria trapped on the p-DEP microelectrode.",
author = "R. Hamada and H. Takayama and Y. Shonishi and L. Mao and Michihiko Nakano and Junya Suehiro",
year = "2013",
month = "3",
day = "25",
doi = "10.1016/j.snb.2013.02.030",
language = "English",
volume = "181",
pages = "439--445",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

TY - JOUR

T1 - A rapid bacteria detection technique utilizing impedance measurement combined with positive and negative dielectrophoresis

AU - Hamada, R.

AU - Takayama, H.

AU - Shonishi, Y.

AU - Mao, L.

AU - Nakano, Michihiko

AU - Suehiro, Junya

PY - 2013/3/25

Y1 - 2013/3/25

N2 - In this study, a bacterial detection technique and device that utilizes advantages of both positive and negative dielectrophoresis (DEP) has been proposed and demonstrated. The device has two microelectrodes, which serve as a bacteria concentrator using negative DEP (n-DEP) and as a bacteria detector using positive DEP (p-DEP), respectively. Bacteria flowing into the device are repelled under action of n-DEP force exerted by the first microelectrode, and are pushed toward the second microelectrode situated at the downstream. Then concentrated bacteria are finally captured by p-DEP on the second microelectrode and detected by dielectrophoretic impedance measurement (DEPIM) method. The numerical simulations and experiments proved that n-DEP concentrator could make DEPIM sensitivity two times higher than that without n-DEP as a result of increased number of bacteria trapped on the p-DEP microelectrode.

AB - In this study, a bacterial detection technique and device that utilizes advantages of both positive and negative dielectrophoresis (DEP) has been proposed and demonstrated. The device has two microelectrodes, which serve as a bacteria concentrator using negative DEP (n-DEP) and as a bacteria detector using positive DEP (p-DEP), respectively. Bacteria flowing into the device are repelled under action of n-DEP force exerted by the first microelectrode, and are pushed toward the second microelectrode situated at the downstream. Then concentrated bacteria are finally captured by p-DEP on the second microelectrode and detected by dielectrophoretic impedance measurement (DEPIM) method. The numerical simulations and experiments proved that n-DEP concentrator could make DEPIM sensitivity two times higher than that without n-DEP as a result of increased number of bacteria trapped on the p-DEP microelectrode.

UR - http://www.scopus.com/inward/record.url?scp=84875155758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875155758&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2013.02.030

DO - 10.1016/j.snb.2013.02.030

M3 - Article

VL - 181

SP - 439

EP - 445

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -