A rapid one-step immunochromatographic assay for the detection of asiaticoside

Boonchoo Sritularak, Thaweesak Juengwatanatrakul, Waraporn Putalun, Hiroyuki Tanaka, Satoshi Morimoto

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Asiaticoside has been identified as the most active compound in Centella asiatica. In order to screen a large number of plant samples for the presence of asiaticoside, a rapid and simple technique is required that utilizes small quantities for test samples. In this study, an immunochromatographic strip test has been developed for the detection of asiaticoside in plant samples that uses a monoclonal antibody against asiaticoside. The limit of detection for the strip test was 12.5 μg/ml. Immunoassay using monoclonal antibodies could be useful for the determination of small quantities of asiaticoside in plant extracts.

元の言語英語
ページ(範囲)279-283
ページ数5
ジャーナルJournal of Natural Medicines
66
発行部数2
DOI
出版物ステータス出版済み - 4 1 2012

Fingerprint

Immunochromatography
Assays
Centella
Monoclonal Antibodies
Plant Extracts
Immunoassay
Limit of Detection
asiaticoside

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

これを引用

A rapid one-step immunochromatographic assay for the detection of asiaticoside. / Sritularak, Boonchoo; Juengwatanatrakul, Thaweesak; Putalun, Waraporn; Tanaka, Hiroyuki; Morimoto, Satoshi.

:: Journal of Natural Medicines, 巻 66, 番号 2, 01.04.2012, p. 279-283.

研究成果: ジャーナルへの寄稿記事

Sritularak, Boonchoo ; Juengwatanatrakul, Thaweesak ; Putalun, Waraporn ; Tanaka, Hiroyuki ; Morimoto, Satoshi. / A rapid one-step immunochromatographic assay for the detection of asiaticoside. :: Journal of Natural Medicines. 2012 ; 巻 66, 番号 2. pp. 279-283.
@article{f09c601afbc04a8fa12566b0ab22ce76,
title = "A rapid one-step immunochromatographic assay for the detection of asiaticoside",
abstract = "Asiaticoside has been identified as the most active compound in Centella asiatica. In order to screen a large number of plant samples for the presence of asiaticoside, a rapid and simple technique is required that utilizes small quantities for test samples. In this study, an immunochromatographic strip test has been developed for the detection of asiaticoside in plant samples that uses a monoclonal antibody against asiaticoside. The limit of detection for the strip test was 12.5 μg/ml. Immunoassay using monoclonal antibodies could be useful for the determination of small quantities of asiaticoside in plant extracts.",
author = "Boonchoo Sritularak and Thaweesak Juengwatanatrakul and Waraporn Putalun and Hiroyuki Tanaka and Satoshi Morimoto",
year = "2012",
month = "4",
day = "1",
doi = "10.1007/s11418-011-0582-2",
language = "English",
volume = "66",
pages = "279--283",
journal = "Journal of Natural Medicines",
issn = "1340-3443",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - A rapid one-step immunochromatographic assay for the detection of asiaticoside

AU - Sritularak, Boonchoo

AU - Juengwatanatrakul, Thaweesak

AU - Putalun, Waraporn

AU - Tanaka, Hiroyuki

AU - Morimoto, Satoshi

PY - 2012/4/1

Y1 - 2012/4/1

N2 - Asiaticoside has been identified as the most active compound in Centella asiatica. In order to screen a large number of plant samples for the presence of asiaticoside, a rapid and simple technique is required that utilizes small quantities for test samples. In this study, an immunochromatographic strip test has been developed for the detection of asiaticoside in plant samples that uses a monoclonal antibody against asiaticoside. The limit of detection for the strip test was 12.5 μg/ml. Immunoassay using monoclonal antibodies could be useful for the determination of small quantities of asiaticoside in plant extracts.

AB - Asiaticoside has been identified as the most active compound in Centella asiatica. In order to screen a large number of plant samples for the presence of asiaticoside, a rapid and simple technique is required that utilizes small quantities for test samples. In this study, an immunochromatographic strip test has been developed for the detection of asiaticoside in plant samples that uses a monoclonal antibody against asiaticoside. The limit of detection for the strip test was 12.5 μg/ml. Immunoassay using monoclonal antibodies could be useful for the determination of small quantities of asiaticoside in plant extracts.

UR - http://www.scopus.com/inward/record.url?scp=84863505584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863505584&partnerID=8YFLogxK

U2 - 10.1007/s11418-011-0582-2

DO - 10.1007/s11418-011-0582-2

M3 - Article

C2 - 21874622

AN - SCOPUS:84863505584

VL - 66

SP - 279

EP - 283

JO - Journal of Natural Medicines

JF - Journal of Natural Medicines

SN - 1340-3443

IS - 2

ER -