A rapid screening and production method using a novel mammalian cell display to isolate human monoclonal antibodies

Kosuke Tomimatsu, Shin Ei Matsumoto, Hayato Tanaka, Makiko Yamashita, Hidekazu Nakanishi, Kiichiro Teruya, Saiko Kazuno, Tomoya Kinjo, Takeki Hamasaki, Ken Ichi Kusumoto, Shigeru Kabayama, Yoshinori Katakura, Sanetaka Shirahata

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

Antibody display methods are increasingly being used to produce human monoclonal antibodies for disease therapy. Rapid screening and isolation of specific human antibody genes are valuable for producing human monoclonal antibodies showing high specificity and affinity. In this report, we describe a novel mammalian cell display method in which whole human IgG is displayed on the cell surface of CHO cells. Cells expressing antigen-specific human monoclonal IgGs with high affinity on the cell surface after normal folding and posttranscriptional modification were screened using a cell sorter. The membrane-type IgG-expressing CHO cells were then converted to IgG-secreting cells by transfection with a plasmid coding Cre recombinase. This mammalian cell display method was applied to in vitro affinity maturation of monoclonal C9 IgG specific to the human high-affinity IgE receptor (FcεRIα). The CDR3 of the C9 heavy chain variable region gene was randomly mutated and inserted into pcDNA5FRT/IgG. A C9 IgG (CDRH3r)-expressing CHO cell display library consisting of 1.1 × 106 independent clones was constructed. IgG-displaying cells showing high reactivity to FcεRIα antigen were screened by the cell sorter, resulting in the establishment of a CHO cell line producing with higher reactivity than the parent C9 IgG.

本文言語英語
ページ(範囲)59-64
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
441
1
DOI
出版ステータス出版済み - 11 8 2013

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「A rapid screening and production method using a novel mammalian cell display to isolate human monoclonal antibodies」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル