A reduction in the number of peripheral CD28+ CD8+ T cells in the acute phase of influenza

S. Nabeshima, M. Murata, K. Kikuchi, H. Ikematsu, S. Kashiwagi, J. Hayashi

研究成果: ジャーナルへの寄稿学術誌査読

18 被引用数 (Scopus)

抄録

Influenza patients show a high incidence of T lymphocytopenia in the acute phase of the illness. Since CD8+ T cells play an important role in influenza virus infection, we investigated which subset of CD8+ T cells was involved in this lymphocytopenia. CD8+ T cells from eight patients with influenza A were studied for lymphocyte count, surface marker, and intracellular IFN-γ production in the acute (days 1-3) and recovery phases (days 9-12). Total and T lymphocyte counts in the acute phase were approximately three times less than in the recovery phase; however, the CD4/8 ratio was the same in both phases. The cell count reduction in the acute phase was attributed predominantly to the CD28+ CD8+ subset, compared with the CD28- CD8+ subset. The memory/activation marker CD45RO on the CD8+ T cells was assessed. The CD28+ CD45RO- subset, a naive phenotype, was reduced significantly in number in the acute phase compared with the recovery phase. The CD28+ CD45RO+ subset, a memory phenotype, was also reduced in the acute phase, but the reduction was not statistically significant. Intracellular IFN-γ in the CD8+ subset after mitogenic stimulation was measured by flow cytometry; the percentage of CD28+ IFN-γ-/CD8+ subset in the acute phase was significantly less than in the recovery phase. These results indicated that the predominant reduction of peripheral CD8+ T cells in the acute phase of influenza was from naive-type lymphocytes, suggesting that these quantitative and qualitative changes of CD8+ T cells in influenza are important for understanding the immunological pathogenesis.

本文言語英語
ページ(範囲)339-346
ページ数8
ジャーナルClinical and Experimental Immunology
128
2
DOI
出版ステータス出版済み - 2002

!!!All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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