A serine protease zymogen functions as a pattern-recognition receptor for lipopolysaccharides

Shigeru Ariki, Kumiko Koori, Tsukasa Osaki, Kiyohito Motoyama, Kei Ichiro Inamori, Shun-Ichiro Kawabata

研究成果: ジャーナルへの寄稿記事

59 引用 (Scopus)

抄録

Bacterial lipopolysaccharide (LPS)-induced exocytosis of granular hemocytes is a key component of the horseshoe crab's innate immunity to infectious microorganisms; stimulation by LPS induces the secretion of various defense molecules from the granular hemocytes. Using a previously uncharacterized assay for exocytosis, we clearly show that hemocytes respond only to LPS and not to other pathogen-associated molecular patterns, such as β-1,3-glucans and peptidoglycans. Furthermore, we show that a granular protein called factor C, an LPS-recognizing serine protease zymogen that initiates the hemolymph coagulation cascade, also exists on the hemocyte surface as a biosensor for LPS. Our data demonstrate that the proteolytic activity of factor C is both necessary and sufficient to trigger exocytosis through a heterotrimeric GTP-binding protein-mediating signaling pathway. Exocytosis of hemocytes was not induced by thrombin, but it was induced by hexapeptides corresponding to the tethered ligands of protease-activated G protein-coupled receptors (PARs). This finding suggested the presence of a PAR-like receptor on the hemocyte surface. We conclude that the serine protease zymogen on the hemocyte surface functions as a pattern-recognition protein for LPS.

元の言語英語
ページ(範囲)953-958
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
101
発行部数4
DOI
出版物ステータス出版済み - 1 27 2004

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Pattern Recognition Receptors
Hemocytes
Enzyme Precursors
Serine Proteases
Lipopolysaccharides
Exocytosis
Heterotrimeric GTP-Binding Proteins
Horseshoe Crabs
Glucans
Hemolymph
Peptidoglycan
Biosensing Techniques
G-Protein-Coupled Receptors
Innate Immunity
Thrombin
Proteins
Peptide Hydrolases
Ligands

All Science Journal Classification (ASJC) codes

  • Genetics
  • General

これを引用

A serine protease zymogen functions as a pattern-recognition receptor for lipopolysaccharides. / Ariki, Shigeru; Koori, Kumiko; Osaki, Tsukasa; Motoyama, Kiyohito; Inamori, Kei Ichiro; Kawabata, Shun-Ichiro.

:: Proceedings of the National Academy of Sciences of the United States of America, 巻 101, 番号 4, 27.01.2004, p. 953-958.

研究成果: ジャーナルへの寄稿記事

Ariki, Shigeru ; Koori, Kumiko ; Osaki, Tsukasa ; Motoyama, Kiyohito ; Inamori, Kei Ichiro ; Kawabata, Shun-Ichiro. / A serine protease zymogen functions as a pattern-recognition receptor for lipopolysaccharides. :: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; 巻 101, 番号 4. pp. 953-958.
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