A simple method of optical evaluation of the distribution of muscle extracellular matrix by immunofluorescence and image analysis using fluorescent semiconductor nanocrystals (quantum dot)

Yoshi Nori Nakamura, Tomiko Fumita, Keisuke Hayashi, Fundo Ebara, Tetsuji Etoh, Yuji Shiotsuka, Masa Aki Hattori, Takafumi Gotoh

研究成果: ジャーナルへの寄稿記事

抄録

A simple method using optically stable quantum dots (Qdots) for immunohistochemical (IHC) analysis was tested to investigate their signal localization and stability in extracellular matrix (ECM) components of bovine longissimus thoracis muscles in fattened Japanese black steers. Detection of primary antibodies of each ECM component in IHC was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide in Qdots with a fluorescence microscope (FM). We could observe each ECM component by IHC using Qdots in a FM for a long time. Each ECM component was detected clearly in the perimysium and endomysium in skeletal muscle. Using computer image analysis software, each ECM component was reconstructed in detail: structures were clear, with the distribution of each component of the ECM shown after 3D conversion based on the strength of fluorescenece. It is possible and useful to apply a simple method of IHC using Qdots under FM to the detailed investigation of ECM components in skeletal muscle.

元の言語英語
ページ(範囲)103-108
ページ数6
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
54
発行部数1
出版物ステータス出版済み - 2 1 2009

Fingerprint

quantum dots
Quantum Dots
extracellular matrix
fluorescent antibody technique
Fluorescent Antibody Technique
Extracellular Matrix
image analysis
Muscles
muscles
Fluorescence
microscopes
fluorescence
skeletal muscle
methodology
Skeletal Muscle
streptavidin
Wagyu
Streptavidin
longissimus muscle
crystals

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

A simple method of optical evaluation of the distribution of muscle extracellular matrix by immunofluorescence and image analysis using fluorescent semiconductor nanocrystals (quantum dot). / Nakamura, Yoshi Nori; Fumita, Tomiko; Hayashi, Keisuke; Ebara, Fundo; Etoh, Tetsuji; Shiotsuka, Yuji; Hattori, Masa Aki; Gotoh, Takafumi.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 54, 番号 1, 01.02.2009, p. 103-108.

研究成果: ジャーナルへの寄稿記事

Nakamura, Yoshi Nori ; Fumita, Tomiko ; Hayashi, Keisuke ; Ebara, Fundo ; Etoh, Tetsuji ; Shiotsuka, Yuji ; Hattori, Masa Aki ; Gotoh, Takafumi. / A simple method of optical evaluation of the distribution of muscle extracellular matrix by immunofluorescence and image analysis using fluorescent semiconductor nanocrystals (quantum dot). :: Journal of the Faculty of Agriculture, Kyushu University. 2009 ; 巻 54, 番号 1. pp. 103-108.
@article{9415701d6af84ae2819c6d838572aab8,
title = "A simple method of optical evaluation of the distribution of muscle extracellular matrix by immunofluorescence and image analysis using fluorescent semiconductor nanocrystals (quantum dot)",
abstract = "A simple method using optically stable quantum dots (Qdots) for immunohistochemical (IHC) analysis was tested to investigate their signal localization and stability in extracellular matrix (ECM) components of bovine longissimus thoracis muscles in fattened Japanese black steers. Detection of primary antibodies of each ECM component in IHC was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide in Qdots with a fluorescence microscope (FM). We could observe each ECM component by IHC using Qdots in a FM for a long time. Each ECM component was detected clearly in the perimysium and endomysium in skeletal muscle. Using computer image analysis software, each ECM component was reconstructed in detail: structures were clear, with the distribution of each component of the ECM shown after 3D conversion based on the strength of fluorescenece. It is possible and useful to apply a simple method of IHC using Qdots under FM to the detailed investigation of ECM components in skeletal muscle.",
author = "Nakamura, {Yoshi Nori} and Tomiko Fumita and Keisuke Hayashi and Fundo Ebara and Tetsuji Etoh and Yuji Shiotsuka and Hattori, {Masa Aki} and Takafumi Gotoh",
year = "2009",
month = "2",
day = "1",
language = "English",
volume = "54",
pages = "103--108",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "1",

}

TY - JOUR

T1 - A simple method of optical evaluation of the distribution of muscle extracellular matrix by immunofluorescence and image analysis using fluorescent semiconductor nanocrystals (quantum dot)

AU - Nakamura, Yoshi Nori

AU - Fumita, Tomiko

AU - Hayashi, Keisuke

AU - Ebara, Fundo

AU - Etoh, Tetsuji

AU - Shiotsuka, Yuji

AU - Hattori, Masa Aki

AU - Gotoh, Takafumi

PY - 2009/2/1

Y1 - 2009/2/1

N2 - A simple method using optically stable quantum dots (Qdots) for immunohistochemical (IHC) analysis was tested to investigate their signal localization and stability in extracellular matrix (ECM) components of bovine longissimus thoracis muscles in fattened Japanese black steers. Detection of primary antibodies of each ECM component in IHC was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide in Qdots with a fluorescence microscope (FM). We could observe each ECM component by IHC using Qdots in a FM for a long time. Each ECM component was detected clearly in the perimysium and endomysium in skeletal muscle. Using computer image analysis software, each ECM component was reconstructed in detail: structures were clear, with the distribution of each component of the ECM shown after 3D conversion based on the strength of fluorescenece. It is possible and useful to apply a simple method of IHC using Qdots under FM to the detailed investigation of ECM components in skeletal muscle.

AB - A simple method using optically stable quantum dots (Qdots) for immunohistochemical (IHC) analysis was tested to investigate their signal localization and stability in extracellular matrix (ECM) components of bovine longissimus thoracis muscles in fattened Japanese black steers. Detection of primary antibodies of each ECM component in IHC was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide in Qdots with a fluorescence microscope (FM). We could observe each ECM component by IHC using Qdots in a FM for a long time. Each ECM component was detected clearly in the perimysium and endomysium in skeletal muscle. Using computer image analysis software, each ECM component was reconstructed in detail: structures were clear, with the distribution of each component of the ECM shown after 3D conversion based on the strength of fluorescenece. It is possible and useful to apply a simple method of IHC using Qdots under FM to the detailed investigation of ECM components in skeletal muscle.

UR - http://www.scopus.com/inward/record.url?scp=63649099640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63649099640&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:63649099640

VL - 54

SP - 103

EP - 108

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 1

ER -