A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates

Yoji Asami, Jun Oishi, Hirotaro Kitazaki, Jumpei Kamimoto, Jeong Hun Kang, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Here we developed a simple set-and-mix assay to perform high-throughput screening of protein kinase A (PKA) inhibitors from the LOPAC 1280 compound library. This assay is based on the color change of gold nanoparticles on aggregation induced by a cationic substrate peptide as coagulant. In spite of the simplicity of this assay system, this assay can be applied to drug screening based on cellular kinases. We successfully found several highly active inhibitors, including compounds that have not been reported before.

元の言語英語
ページ(範囲)44-49
ページ数6
ジャーナルAnalytical Biochemistry
418
発行部数1
DOI
出版物ステータス出版済み - 11 1 2011

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Coagulants
Preclinical Drug Evaluations
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
Gold
Nanoparticles
Libraries
Assays
Screening
Phosphotransferases
Color
Peptides
Agglomeration
Throughput
Substrates
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates. / Asami, Yoji; Oishi, Jun; Kitazaki, Hirotaro; Kamimoto, Jumpei; Kang, Jeong Hun; Niidome, Takuro; Mori, Takeshi; Katayama, Yoshiki.

:: Analytical Biochemistry, 巻 418, 番号 1, 01.11.2011, p. 44-49.

研究成果: ジャーナルへの寄稿記事

Asami Y, Oishi J, Kitazaki H, Kamimoto J, Kang JH, Niidome T その他. A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates. Analytical Biochemistry. 2011 11 1;418(1):44-49. https://doi.org/10.1016/j.ab.2011.06.041
Asami, Yoji ; Oishi, Jun ; Kitazaki, Hirotaro ; Kamimoto, Jumpei ; Kang, Jeong Hun ; Niidome, Takuro ; Mori, Takeshi ; Katayama, Yoshiki. / A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates. :: Analytical Biochemistry. 2011 ; 巻 418, 番号 1. pp. 44-49.
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