A Simulation Study of Light Propagation in the Spinal Cord for Optogenetic Surface Stimulation

Shih Yin Chang, Satoshi Nishikawa, Masaki Sekino, Hiroshi Onodera, Yasuo Kuniyoshi

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

For utilizing optogenetics in neuroscience research, a proper setup is necessary, which delivers sufficient light to target cells and minimizes unexpected side effects caused by light exposure. In this study, we were interested in the area of influence of optical surface stimulation on a spinal cord tissue. We built a 3D spinal cord structure of rat and utilized the Monte-Carlo methods to simulate the light transport in it. We first evaluated light propagation in homogeneous nervous tissue models. For a 10-mW, 470-nm light source, light intensity of 1 mW=mm2 was detected at depths of 1:14 and 1:77 mm in white and grey matter, respectively. This indicated a narrower spreading pattern of light in the white matter than in the grey matter. Since the grey matter, which contains the somatosensory pathways, is an important target of spinal cord stimulation, we focused on investigating how much light could reach this area in a multi-layered structure. The results showed that when an optical fiber was positioned in the center line of the spinal cord dorsal surface, most of the light energy was absorbed before reaching the grey matter. In contrast, when we put the fiber on a lateral position, 0:8mm away from the central line, relatively sufficient light intensity could be detected deep into the lamina 5 area. The experimental results obtained herein suggest that tissue type and the position of stimulation could greatly affect the area of influence of light stimulation in a 3D spinal cord. It is important to consider the location of the interested neural pathways and plan a proper stimulation site before conducting optogenetic surface stimulation of the spinal cord.

本文言語英語
ホスト出版物のタイトル2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
出版社Institute of Electrical and Electronics Engineers Inc.
ページ6872-6875
ページ数4
ISBN(電子版)9781538613115
DOI
出版ステータス出版済み - 7 2019
外部発表はい
イベント41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019 - Berlin, ドイツ
継続期間: 7 23 20197 27 2019

出版物シリーズ

名前Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN(印刷版)1557-170X

会議

会議41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2019
国/地域ドイツ
CityBerlin
Period7/23/197/27/19

All Science Journal Classification (ASJC) codes

  • 信号処理
  • 生体医工学
  • コンピュータ ビジョンおよびパターン認識
  • 健康情報学

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