A single cell culture system using lectin-conjugated magnetite nanoparticles and magnetic force to screen mutant cyanobacteria

Sayuri Arai, Mina Okochi, Kazunori Shimizu, Taizo Hanai, Hiroyuki Honda

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Cyanobacteria can be utilized as a potential biocatalyst for the production of biofuels and biochemicals directly from CO2. Useful mutants of cyanobacteria, which can grow rapidly or are resistant to specific metabolic products, are essential to improve the productivity of biofuels. In this study, we developed a single cell culture system to effectively screen mutant cyanobacteria using magnetite nanoparticles and magnetic force. Lens culinaris Agglutinin (LCA) was selected as a lectin, which binds to the surface of Synechococcus elongatus PCC7942 cells and the LCA-conjugated magnetite cationic liposomes (MCLs) were developed for magnetic labeling of PCC7942 cells. The MCL-labeled PCC7942 cells were magnetically patterned at a single cell level by using 6,400 iron pillars of the pin-holder device. The device enabled 1,600 single cells to be arrayed in one square centimeter. We cultured the patterned cells in liquid medium and achieved higher colony-forming ratio (78.4%) than that obtained using conventional solid culture method (4.8%). Single cells with different properties could be distinguished in the single cell culture system depending on their growth. Furthermore, we could selectively pick up the target cells and subsequently perform efficient isolation culture. The ratio of successful isolation culture using the developed method was 13 times higher than that of the conventional methods. Thus, the developed system would serve as a powerful tool for screening mutant cyanobacteria.

元の言語英語
ページ(範囲)112-119
ページ数8
ジャーナルBiotechnology and Bioengineering
113
発行部数1
DOI
出版物ステータス出版済み - 1 1 2016

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Magnetite Nanoparticles
Magnetite nanoparticles
Cyanobacteria
Cell culture
Lectins
Cell Culture Techniques
Ferrosoferric Oxide
Biofuels
Liposomes
Magnetite
Lenses
Biocatalysts
Labeling
Screening
Iron
Synechococcus
Productivity
Equipment and Supplies
Liquids
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

A single cell culture system using lectin-conjugated magnetite nanoparticles and magnetic force to screen mutant cyanobacteria. / Arai, Sayuri; Okochi, Mina; Shimizu, Kazunori; Hanai, Taizo; Honda, Hiroyuki.

:: Biotechnology and Bioengineering, 巻 113, 番号 1, 01.01.2016, p. 112-119.

研究成果: ジャーナルへの寄稿記事

Arai, Sayuri ; Okochi, Mina ; Shimizu, Kazunori ; Hanai, Taizo ; Honda, Hiroyuki. / A single cell culture system using lectin-conjugated magnetite nanoparticles and magnetic force to screen mutant cyanobacteria. :: Biotechnology and Bioengineering. 2016 ; 巻 113, 番号 1. pp. 112-119.
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