A small chimerically bifunctional monomeric protein: Tapes japonica lysozyme

K. Takeshita, Y. Hashimoto, Tadashi Ueda, T. Imoto

研究成果: ジャーナルへの寄稿学術誌査読

36 被引用数 (Scopus)

抄録

The lysozyme of the marine bilave Tapes japonica (13.8 kDa) is a novel protein. The protein has 46% homology with the destabilase from medicinal leech that has isopeptidase activity. Based on these data, we confirmed hydrolysis activity of T. japonica lysozyme against three substrates: L-γ-Glu-pNA, D-γ-Glu-pNA, and ε-(γ-Glu)-L-Lys. The optimal pH of chitinase and isopeptidase activity was 5.0 and 7.0, respectively. The isopeptidase activity was inhibited with serine protease inhibitor, but the lytic and chitinase activities were not. Moreover, only isopeptidase activity is decreased by lyophilization, but lytic and chitinase activities were not. We conclude that T. japonica lysozyme expresses isopeptidase and chitinase activity at different active sites.

本文言語英語
ページ(範囲)1944-1951
ページ数8
ジャーナルCellular and Molecular Life Sciences
60
9
DOI
出版ステータス出版済み - 9月 1 2003

!!!All Science Journal Classification (ASJC) codes

  • 分子医療
  • 分子生物学
  • 薬理学
  • 細胞および分子神経科学
  • 細胞生物学

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