A small modulatory dsRNA specifies the fate of adult neural stem cells

Tomoko Kuwabara, Jenny Hsieh, Kinichi Nakashima, Kazunari Taira, Fred H. Gage

研究成果: ジャーナルへの寄稿記事

356 引用 (Scopus)

抄録

Discovering the molecular mechanisms that regulate neuron-specific gene expression remains a central challenge for CNS research. Here, we report that small, noncoding double-stranded (ds) RNAs play a critical role in mediating neuronal differentiation. The sequence defined by this dsRNA is NRSE/RE1, which is recognized by NRSF/REST, known primarily as a negative transcriptional regulator that restricts neuronal gene expression to neurons. The NRSE dsRNA can trigger gene expression of neuron-specific genes through interaction with NRSF/REST transcriptional machinery, resulting in the transition from neural stem cells with neuron-specific genes silenced by NRSF/REST into cells with neuronal identity that can express neuronal genes. The mechanism of action appears to be mediated through a dsRNA/protein interaction, rather than through siRNA or miRNA. The discovery of small modulatory dsRNAs (smRNAs) extends the important contribution of noncoding RNAs as key regulators of cell behavior at both transcriptional and posttranscriptional levels.

元の言語英語
ページ(範囲)779-793
ページ数15
ジャーナルCell
116
発行部数6
DOI
出版物ステータス出版済み - 3 19 2004
外部発表Yes

Fingerprint

Adult Stem Cells
Neural Stem Cells
Stem cells
Neurons
Gene expression
Untranslated RNA
Genes
Gene Expression
Double-Stranded RNA
MicroRNAs
Small Interfering RNA
Machinery
Research
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

A small modulatory dsRNA specifies the fate of adult neural stem cells. / Kuwabara, Tomoko; Hsieh, Jenny; Nakashima, Kinichi; Taira, Kazunari; Gage, Fred H.

:: Cell, 巻 116, 番号 6, 19.03.2004, p. 779-793.

研究成果: ジャーナルへの寄稿記事

Kuwabara, Tomoko ; Hsieh, Jenny ; Nakashima, Kinichi ; Taira, Kazunari ; Gage, Fred H. / A small modulatory dsRNA specifies the fate of adult neural stem cells. :: Cell. 2004 ; 巻 116, 番号 6. pp. 779-793.
@article{04a900285c9245eaa4e3fb34dd66c794,
title = "A small modulatory dsRNA specifies the fate of adult neural stem cells",
abstract = "Discovering the molecular mechanisms that regulate neuron-specific gene expression remains a central challenge for CNS research. Here, we report that small, noncoding double-stranded (ds) RNAs play a critical role in mediating neuronal differentiation. The sequence defined by this dsRNA is NRSE/RE1, which is recognized by NRSF/REST, known primarily as a negative transcriptional regulator that restricts neuronal gene expression to neurons. The NRSE dsRNA can trigger gene expression of neuron-specific genes through interaction with NRSF/REST transcriptional machinery, resulting in the transition from neural stem cells with neuron-specific genes silenced by NRSF/REST into cells with neuronal identity that can express neuronal genes. The mechanism of action appears to be mediated through a dsRNA/protein interaction, rather than through siRNA or miRNA. The discovery of small modulatory dsRNAs (smRNAs) extends the important contribution of noncoding RNAs as key regulators of cell behavior at both transcriptional and posttranscriptional levels.",
author = "Tomoko Kuwabara and Jenny Hsieh and Kinichi Nakashima and Kazunari Taira and Gage, {Fred H.}",
year = "2004",
month = "3",
day = "19",
doi = "10.1016/S0092-8674(04)00248-X",
language = "English",
volume = "116",
pages = "779--793",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - A small modulatory dsRNA specifies the fate of adult neural stem cells

AU - Kuwabara, Tomoko

AU - Hsieh, Jenny

AU - Nakashima, Kinichi

AU - Taira, Kazunari

AU - Gage, Fred H.

PY - 2004/3/19

Y1 - 2004/3/19

N2 - Discovering the molecular mechanisms that regulate neuron-specific gene expression remains a central challenge for CNS research. Here, we report that small, noncoding double-stranded (ds) RNAs play a critical role in mediating neuronal differentiation. The sequence defined by this dsRNA is NRSE/RE1, which is recognized by NRSF/REST, known primarily as a negative transcriptional regulator that restricts neuronal gene expression to neurons. The NRSE dsRNA can trigger gene expression of neuron-specific genes through interaction with NRSF/REST transcriptional machinery, resulting in the transition from neural stem cells with neuron-specific genes silenced by NRSF/REST into cells with neuronal identity that can express neuronal genes. The mechanism of action appears to be mediated through a dsRNA/protein interaction, rather than through siRNA or miRNA. The discovery of small modulatory dsRNAs (smRNAs) extends the important contribution of noncoding RNAs as key regulators of cell behavior at both transcriptional and posttranscriptional levels.

AB - Discovering the molecular mechanisms that regulate neuron-specific gene expression remains a central challenge for CNS research. Here, we report that small, noncoding double-stranded (ds) RNAs play a critical role in mediating neuronal differentiation. The sequence defined by this dsRNA is NRSE/RE1, which is recognized by NRSF/REST, known primarily as a negative transcriptional regulator that restricts neuronal gene expression to neurons. The NRSE dsRNA can trigger gene expression of neuron-specific genes through interaction with NRSF/REST transcriptional machinery, resulting in the transition from neural stem cells with neuron-specific genes silenced by NRSF/REST into cells with neuronal identity that can express neuronal genes. The mechanism of action appears to be mediated through a dsRNA/protein interaction, rather than through siRNA or miRNA. The discovery of small modulatory dsRNAs (smRNAs) extends the important contribution of noncoding RNAs as key regulators of cell behavior at both transcriptional and posttranscriptional levels.

UR - http://www.scopus.com/inward/record.url?scp=1642603947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642603947&partnerID=8YFLogxK

U2 - 10.1016/S0092-8674(04)00248-X

DO - 10.1016/S0092-8674(04)00248-X

M3 - Article

C2 - 15035981

AN - SCOPUS:1642603947

VL - 116

SP - 779

EP - 793

JO - Cell

JF - Cell

SN - 0092-8674

IS - 6

ER -