A solid-in-oil-in-water emulsion: An adjuvant-based immune-carrier enhances vaccine effect

Yoshiro Tahara, Riko Mizuno, Tomoki Nishimura, Sada atsu Mukai, Rie Wakabayashi, Noriho Kamiya, Kazunari Akiyoshi, Masahiro Goto

研究成果: ジャーナルへの寄稿学術誌査読

抄録

The biomaterial-based immunoengineering has become one of the most attractive research fields in the last decade. In the present study, a solid-in-oil-in-water (S/O/W) emulsion encapsulating antigen in the oil phase of an oil-in-water (O/W) emulsion was prepared as a novel vaccine carrier consisting of similar materials to the emulsion adjuvant of which the safety, immunogenicity and vaccination efficacy have been already confirmed in human. Direct observation by high-resolution confocal laser scanning microscopy and small angle X-ray scattering analysis showed that the antigens were dispersed inside of the oil phase of the S/O/W emulsion as solid-state particles. The S/O/W emulsion robustly produced antigen-specific antibodies and enhanced the antitumor effects in a therapeutic cancer vaccination compared with free antigens or the O/W emulsion in vivo. This result is in good agreement with the activation effect of antigen-specific cytotoxic T lymphocytes and antigen presentation by the S/O/W emulsion, indicating that the S/O/W emulsion consisting of already approved materials is a promising vaccine carrier to produce both humoral and cellular immunity.

本文言語英語
論文番号121385
ジャーナルBiomaterials
282
DOI
出版ステータス出版済み - 3月 2022

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • バイオエンジニアリング
  • セラミックおよび複合材料
  • 生体材料
  • 材料力学

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