A structural model of the PriB–DnaT complex in Escherichia coli replication restart

Yoshito Abe, Yohei Ikeda, Saki Fujiyama, R. Manjunatha Kini, Tadashi Ueda

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

In Escherichia coli, DNA replication is restarted following DNA repair by the PriA-dependent pathway, in which the binding and dissociation of proteins such as PriA, PriB, and DnaT on ssDNA lead to the formation of a protein–DNA complex for recruiting the DnaB–DnaC replication protein complex. However, the structure of the PriB–DnaT complex, which is an essential step in the PriA-dependent pathway, remains elusive. In this study, the importance of His26 in PriB for replication restart was reconfirmed using plasmid complementation. Furthermore, we used NMR to examine the DnaT interaction sites on PriB. We also evaluated the PriB–DnaT peptide complex model, which was prepared by in silico docking, using molecular dynamic simulation. From these data, we propose a structural model that provides insight into the PriB–DnaT interaction.

本文言語英語
ページ(範囲)341-350
ページ数10
ジャーナルFEBS Letters
595
3
DOI
出版ステータス出版済み - 2月 2021

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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