A study of boronic acid based fluorescent glucose sensors

T. Kawanishi, M. A. Romey, P. C. Zhu, M. Z. Holody, S. Shinkai

研究成果: Contribution to journalArticle査読

65 被引用数 (Scopus)

抄録

Boronic acid based anthracene dyes were designed, synthesized, and immobilized to solid phase, creating a continuous glucose sensor. Glucose sensitivities of dyes can decrease drastically after immobilization, therefore how to immobilize a dye to solid phase without changing the dye property is a key issue in developing the sensor. The glucose sensitivity of the simplest 1st generation sensor, which is based on an immobilized mono-phenylboronate/single- arm type, came short of the sensitivity requirement for practical use, because of the very moderate fluorescence intensity change over the physiological glucose range. However, the 2nd generation, an immobilized bis-phenylboronate/ double-arm type sensor, which contained two boronate groups in the dye moiety in expectation of a large intensity change, brought about considerable improvement on its glucose sensitivity. We tried to introduce functional groups onto an anthracene ring in order to improve the dies' fluorescence properties. Acetyl or carboxyl substitution on anthracene contributed to shift the fluorescence wavelength into the more visible range (red-shift) and a divergence of wavelength between an excitation peak and an emission peak. This improvement is advantageous to the design of an optical detection system. Furthermore, single arm immobilization to this carboxyl group, thus linking directly to the fluorophore led to a 3rd generation sensor, an immobilized bis-phenylboronate/ single-arm type, that was twice as sensitive as that of the 2nd generation sensor, presumably due to increased mobility of the dye moiety. The results of our study advance closer toward a clinically useful continuous fluorescent glucose sensor.

本文言語英語
ページ(範囲)499-512
ページ数14
ジャーナルJournal of Fluorescence
14
5
DOI
出版ステータス出版済み - 9 2004

All Science Journal Classification (ASJC) codes

  • 生化学
  • 臨床心理学
  • 社会科学(その他)
  • 社会学および政治科学
  • 分光学
  • 臨床生化学
  • 法学

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