A Study of Cytochrome c Oxidase in Lipid Bilayer Membranes on Electrode Surfaces

John K. Cullison, Fred M. Hawkridge, Naotoshi Nakashima, Shimichi Yoshikawa

    研究成果: Contribution to journalArticle査読

    88 被引用数 (Scopus)

    抄録

    A membrane-associated enzyme, cytochrome c oxidase, has been put in lipid bilayer membranes attached to gold electrode surfaces. Gold/thiol self-assembly chemistry and deoxycholate dialysis procedures have been used to insert cytochrome c oxidase into a lipid bilayer on gold surfaces with a controlled orientation. Voltammetric and spectroelectrochemical results indicate that direct electron-transfer communication between the gold electrode surface and cytochrome c oxidase has been achieved. Moreover, immobilized cytochrome c oxidase can both reduce and oxidize solution-resident cytochrome c by appropriately controlling the applied electrode potential.

    本文言語英語
    ページ(範囲)877-882
    ページ数6
    ジャーナルLangmuir
    10
    3
    DOI
    出版ステータス出版済み - 3 1 1994

    All Science Journal Classification (ASJC) codes

    • 材料科学(全般)
    • 凝縮系物理学
    • 表面および界面
    • 分光学
    • 電気化学

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