A surface plasmon resonance sensor on a compact disk-type microfluidic device

Akihide Hemmi, Takashi Usui, Akihiro Moto, Tatsuya Tobita, Nobuaki Soh, Koji Nakano, Hulie Zeng, Katsumi Uchiyama, Toshihiko Imato, Hizuru Nakajima

研究成果: Contribution to journalArticle査読

22 被引用数 (Scopus)

抄録

A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.

本文言語英語
ページ(範囲)2913-2919
ページ数7
ジャーナルJournal of Separation Science
34
20
DOI
出版ステータス出版済み - 10 2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学
  • ろ過および分離

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