A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

Hirokazu Suzuki, Keisuke Wada, Megumi Furukawa, Katsumi Doi, Toshihisa Ohshima

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

元の言語英語
ページ(範囲)2316-2318
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
77
発行部数11
DOI
出版物ステータス出版済み - 12 6 2013

Fingerprint

Geobacillus
Gene Library
Orotidine-5'-Phosphate Decarboxylase
Genes
Libraries
Screening
Plasmids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426. / Suzuki, Hirokazu; Wada, Keisuke; Furukawa, Megumi; Doi, Katsumi; Ohshima, Toshihisa.

:: Bioscience, Biotechnology and Biochemistry, 巻 77, 番号 11, 06.12.2013, p. 2316-2318.

研究成果: ジャーナルへの寄稿記事

Suzuki, Hirokazu ; Wada, Keisuke ; Furukawa, Megumi ; Doi, Katsumi ; Ohshima, Toshihisa. / A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426. :: Bioscience, Biotechnology and Biochemistry. 2013 ; 巻 77, 番号 11. pp. 2316-2318.
@article{1e63fa4e0f414d0c8c25223dac8edb25,
title = "A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426",
abstract = "This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.",
author = "Hirokazu Suzuki and Keisuke Wada and Megumi Furukawa and Katsumi Doi and Toshihisa Ohshima",
year = "2013",
month = "12",
day = "6",
doi = "10.1271/bbb.130492",
language = "English",
volume = "77",
pages = "2316--2318",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "11",

}

TY - JOUR

T1 - A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

AU - Suzuki, Hirokazu

AU - Wada, Keisuke

AU - Furukawa, Megumi

AU - Doi, Katsumi

AU - Ohshima, Toshihisa

PY - 2013/12/6

Y1 - 2013/12/6

N2 - This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

AB - This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

UR - http://www.scopus.com/inward/record.url?scp=84888867358&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888867358&partnerID=8YFLogxK

U2 - 10.1271/bbb.130492

DO - 10.1271/bbb.130492

M3 - Article

C2 - 24200788

AN - SCOPUS:84888867358

VL - 77

SP - 2316

EP - 2318

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 11

ER -