TY - JOUR
T1 - A tobacco microsomal ω-3 fatty acid desaturase gene increases the linolenic acid content in transgenic sweet potato (Ipomoea batatas)
AU - Wakita, Y.
AU - Otani, M.
AU - Hamada, T.
AU - Mori, M.
AU - Iba, K.
AU - Shimada, T.
N1 - Funding Information:
Acknowledgements We are grateful to Dr. Yuko Ohashi (National Institute of Agrobiological Resources, Japan) for providing the plasmid pE2113-GUS, and Mr. Takashi Kimura (Kyusyu National Agricultural Experiment Station, Japan) for providing the plasmid SacI cassette. We also thank Mr. Takashi Ozawa for his excellent technical assistance. This work was supported by Grant No. RFTF96L00602 from the Japan Society for Promotion of Science.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - A tobacco microsomal ω-3 fatty acid desaturase gene (NtFAD3) under the control of the CaMV 35S promoter or an improved CaMV 35S promoter (E12ω) was introduced into sweet potato. Transformed sweet potato plants were obtained from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. The transgenic plants grew normally to form storage roots and showed properties similar to those of the non-transgenic plants. The fatty acid composition in the transgenic line with a NtFAD3 gene driven by the CaMV 35S promoter was similar to that in the non-transformant. However, in the transgenic line that had a NtFAD3 gene driven by the E12ω promoter, linoleic acid (18:2) and linolenic acid (18:3) contents were 47.7 mol% and 24.8 mol%, respectively, which were significantly different from the 53.6 mol% and 11.3 mol%, respectively, in the non-transformant. The NtFAD3 gene driven by the E12ω promoter was expressed more strongly than that driven by the CaMV 35S promoter, thereby increasing the linolenic acid content in the transgenic sweet potato plants.
AB - A tobacco microsomal ω-3 fatty acid desaturase gene (NtFAD3) under the control of the CaMV 35S promoter or an improved CaMV 35S promoter (E12ω) was introduced into sweet potato. Transformed sweet potato plants were obtained from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. The transgenic plants grew normally to form storage roots and showed properties similar to those of the non-transgenic plants. The fatty acid composition in the transgenic line with a NtFAD3 gene driven by the CaMV 35S promoter was similar to that in the non-transformant. However, in the transgenic line that had a NtFAD3 gene driven by the E12ω promoter, linoleic acid (18:2) and linolenic acid (18:3) contents were 47.7 mol% and 24.8 mol%, respectively, which were significantly different from the 53.6 mol% and 11.3 mol%, respectively, in the non-transformant. The NtFAD3 gene driven by the E12ω promoter was expressed more strongly than that driven by the CaMV 35S promoter, thereby increasing the linolenic acid content in the transgenic sweet potato plants.
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U2 - 10.1007/s002990100316
DO - 10.1007/s002990100316
M3 - Article
AN - SCOPUS:0035045205
SN - 0721-7714
VL - 20
SP - 244
EP - 249
JO - Plant Cell Reports
JF - Plant Cell Reports
IS - 3
ER -