A Transcriptional Switch Point during Hematopoietic Stem and Progenitor Cell Ontogeny

Daisuke Sugiyama, Anagha Joshi, Kasem Kulkeaw, Keai Sinn Tan, Tomoko Yokoo-Inoue, Chiyo Mizuochi-Yanagi, Kaori Yasuda, Atsushi Doi, Tadafumi Iino, Masayoshi Itoh, Sayaka Nagao-Sato, Kenzaburo Tani, Koichi Akashi, Yoshihide Hayashizaki, Harukazu Suzuki, Hideya Kawaji, Piero Carninci, Alistair R.R. Forrest

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

During mammalian embryogenesis, hematopoietic stem and progenitor cells (HSPCs) originate from mesoderm-derived endothelial cells in the aorta-gonad-mesonephros (AGM) region and placenta (PL). Later, HSPCs expand in fetal liver (FL) and migrate to bone marrow (BM) shortly before birth. Understanding global transcriptional regulation governing HSPC emergence from embryonic stem/induced pluripotent stem cells is necessary to devise clinical applications, such as novel transplantation approaches. In this study, to assess transcriptional dynamics during development, we performed cap analysis of gene expression on 10 developmental murine HSPC populations isolated from the AGM region, PL, FL, and BM and identified 15,681 transcripts across HSPC ontogeny. We performed microarray analysis of AGM-derived HSPCs at 9.5 and 10.5 days postcoitum (dpc) and identified 40 differentially expressed genes, 23 confirmed as significantly changed by real-time polymerase chain reaction. We conclude that a transcriptional switch point occurs in HSPC ontogeny between 9.5 and 10.5 dpc in the AGM region.

本文言語英語
ページ(範囲)314-327
ページ数14
ジャーナルStem cells and development
26
5
DOI
出版ステータス出版済み - 3 1 2017

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

フィンガープリント 「A Transcriptional Switch Point during Hematopoietic Stem and Progenitor Cell Ontogeny」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル