抄録
Serum response factor and the (CC(A/T)6GG) (CArG) box interact to promote the transcription of c-fos and muscle genes; this tissue-specific activity may require co-regulators for serum response factor. The α1 integrin promoter contains two cis-elements besides the CArG box: a TAAT sequence, a consensus binding site for homeoproteins, and a GATA-binding box. As a candidate TAAT-binding factor, we cloned an NK family homeobox gene, Nkx-3.2, which is expressed mainly in smooth muscle tissues and skeletal structures. Nkx-3.2, serum response factor, and GATA-6 were co-expressed only in the medial smooth muscle layer of arteries. These three transcription factors formed a complex with their corresponding cis-elements and cooperatively transactivated smooth muscle genes, including α1 integrin, SM22α, and caldesmon. Cardiac muscle-specific members of the NK and GATA families exist, and the triad of Nkx-2.5, serum response factor, and GATA-4 also transactivated the cardiac atrial natriuretic factor gene, which contains a CArG-like box, a GATA-binding box, and an NK-binding element. Our findings demonstrate that smooth and cardiac muscle have a shared transcriptional machinery and that the GATA and NK families confer muscle specificity on the serum response factor/CArG interaction.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 7308-7317 |
| ページ数 | 10 |
| ジャーナル | Journal of Biological Chemistry |
| 巻 | 277 |
| 発行部数 | 9 |
| DOI | |
| 出版物ステータス | 出版済み - 3 1 2002 |
| 外部発表 | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Cell Biology
これを引用
A triad of serum response factor and the GATA and NK families governs the transcription of smooth and cardiac muscle genes. / Nishida, Wataru; Nakamura, Mako; Mori, Syunsuke; Takahashi, Masanori; Ohkawa, Yasuyuki; Tadokoro, Satoko; Yoshida, Kenji; Hiwada, Kunio; Hayashi, Ke N.Ichiro; Sobue, Kenji.
：: Journal of Biological Chemistry, 巻 277, 番号 9, 01.03.2002, p. 7308-7317.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - A triad of serum response factor and the GATA and NK families governs the transcription of smooth and cardiac muscle genes
AU - Nishida, Wataru
AU - Nakamura, Mako
AU - Mori, Syunsuke
AU - Takahashi, Masanori
AU - Ohkawa, Yasuyuki
AU - Tadokoro, Satoko
AU - Yoshida, Kenji
AU - Hiwada, Kunio
AU - Hayashi, Ke N.Ichiro
AU - Sobue, Kenji
PY - 2002/3/1
Y1 - 2002/3/1
N2 - Serum response factor and the (CC(A/T)6GG) (CArG) box interact to promote the transcription of c-fos and muscle genes; this tissue-specific activity may require co-regulators for serum response factor. The α1 integrin promoter contains two cis-elements besides the CArG box: a TAAT sequence, a consensus binding site for homeoproteins, and a GATA-binding box. As a candidate TAAT-binding factor, we cloned an NK family homeobox gene, Nkx-3.2, which is expressed mainly in smooth muscle tissues and skeletal structures. Nkx-3.2, serum response factor, and GATA-6 were co-expressed only in the medial smooth muscle layer of arteries. These three transcription factors formed a complex with their corresponding cis-elements and cooperatively transactivated smooth muscle genes, including α1 integrin, SM22α, and caldesmon. Cardiac muscle-specific members of the NK and GATA families exist, and the triad of Nkx-2.5, serum response factor, and GATA-4 also transactivated the cardiac atrial natriuretic factor gene, which contains a CArG-like box, a GATA-binding box, and an NK-binding element. Our findings demonstrate that smooth and cardiac muscle have a shared transcriptional machinery and that the GATA and NK families confer muscle specificity on the serum response factor/CArG interaction.
AB - Serum response factor and the (CC(A/T)6GG) (CArG) box interact to promote the transcription of c-fos and muscle genes; this tissue-specific activity may require co-regulators for serum response factor. The α1 integrin promoter contains two cis-elements besides the CArG box: a TAAT sequence, a consensus binding site for homeoproteins, and a GATA-binding box. As a candidate TAAT-binding factor, we cloned an NK family homeobox gene, Nkx-3.2, which is expressed mainly in smooth muscle tissues and skeletal structures. Nkx-3.2, serum response factor, and GATA-6 were co-expressed only in the medial smooth muscle layer of arteries. These three transcription factors formed a complex with their corresponding cis-elements and cooperatively transactivated smooth muscle genes, including α1 integrin, SM22α, and caldesmon. Cardiac muscle-specific members of the NK and GATA families exist, and the triad of Nkx-2.5, serum response factor, and GATA-4 also transactivated the cardiac atrial natriuretic factor gene, which contains a CArG-like box, a GATA-binding box, and an NK-binding element. Our findings demonstrate that smooth and cardiac muscle have a shared transcriptional machinery and that the GATA and NK families confer muscle specificity on the serum response factor/CArG interaction.
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U2 - 10.1074/jbc.M111824200
DO - 10.1074/jbc.M111824200
M3 - Article
VL - 277
SP - 7308
EP - 7317
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 9
ER -