TY - JOUR
T1 - A uniaxially oriented nanofibrous cellulose scaffold from pellicles produced by Gluconacetobacter xylinus in dissolved oxygen culture
AU - Nagashima, Aya
AU - Tsuji, Tsubasa
AU - Kondo, Tetsuo
N1 - Funding Information:
The authors thank Mr. Eiji Togawa of the Forestry and Forest Products Research Institute (FFPRI) for conducting the WAXD and CP/MAS 13 C NMR measurements. The authors also are indebted to Mr. Ryunosuke Funahashi of an undergraduate student at Kyushu University for his helpful contribution to the experiments. This study is partly supported by Grants-in-Aid for Scientific Research (Nos. 22380097 & 23658146 ) from the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - An aerobic, Gram-negative bacterium, Gluconacetobacter xylinus, was successfully employed to produce a stretchable cellulose nanofiber pellicle using dissolved oxygen in a conventional cultured medium. The obtained nanofibers were highly crystalline with the metastable cellulose Iα phase being apparently the dominant phase by more than 90%. The obtained pellicle could be stretched by up to 1.5 times to provide oriented crystalline nanofibrous films. Low heating of the nanofibrous film induced the transformation of the dominant cellulose Iα crystalline phase into the Iβ crystalline phase without a loss of crystallinity or the high Young's modulus. The film also exhibited unique and anisotropic viscoelastic and mechanical properties as well as superior thermal stability compared with conventional high-performance synthetic polymeric materials. In addition, when G. xylinus cells were transferred to the oriented surface after stretched, they started to synthesize cellulose ribbons that parallel the nanofiber orientation of the substrate. This function as a template was evidenced by direct video imaging of the motion of the bacteria. The application of a bacterial culture using dissolved oxygen in the medium offers the fabrication of novel anisotropic and nanofibrous scaffold of cellulose Iα.
AB - An aerobic, Gram-negative bacterium, Gluconacetobacter xylinus, was successfully employed to produce a stretchable cellulose nanofiber pellicle using dissolved oxygen in a conventional cultured medium. The obtained nanofibers were highly crystalline with the metastable cellulose Iα phase being apparently the dominant phase by more than 90%. The obtained pellicle could be stretched by up to 1.5 times to provide oriented crystalline nanofibrous films. Low heating of the nanofibrous film induced the transformation of the dominant cellulose Iα crystalline phase into the Iβ crystalline phase without a loss of crystallinity or the high Young's modulus. The film also exhibited unique and anisotropic viscoelastic and mechanical properties as well as superior thermal stability compared with conventional high-performance synthetic polymeric materials. In addition, when G. xylinus cells were transferred to the oriented surface after stretched, they started to synthesize cellulose ribbons that parallel the nanofiber orientation of the substrate. This function as a template was evidenced by direct video imaging of the motion of the bacteria. The application of a bacterial culture using dissolved oxygen in the medium offers the fabrication of novel anisotropic and nanofibrous scaffold of cellulose Iα.
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U2 - 10.1016/j.carbpol.2015.08.077
DO - 10.1016/j.carbpol.2015.08.077
M3 - Article
C2 - 26453871
AN - SCOPUS:84941730153
VL - 135
SP - 215
EP - 224
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
ER -