A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions

Shu Ying Feng, Kazuhisa Ota, Yoichi Yamada, Norio Sawabu, Takashi Ito

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

We developed a method for site-selective CpG methylation of the budding yeast genome. The method recruits LexA-fused M.SssI DNA methyltransferase to LexA operator sequences integrated adjacent to the target site. Microarray analysis of methylated DNAs indicated that the tethered enzyme selectively methylates the region around the target site. Exploiting this method to methylate bait DNA in the one-hybrid system, we demonstrated methylation-dependent DNA binding of methyl-CpG binding proteins, MBD1 and Kaiso, in vivo. This methylation-dependent one-hybrid system would provide a versatile tool for the search and analysis of proteins that recognize methylated DNA to participate in epigenetic regulation.

元の言語英語
ページ(範囲)922-925
ページ数4
ジャーナルBiochemical and Biophysical Research Communications
313
発行部数4
DOI
出版物ステータス出版済み - 1 23 2004

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Methylation
DNA Methylation
Hybrid systems
Yeast
Yeasts
DNA
Proteins
Saccharomycetales
Methyltransferases
Microarray Analysis
Epigenomics
Microarrays
Carrier Proteins
Genome
Genes
Enzymes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions. / Feng, Shu Ying; Ota, Kazuhisa; Yamada, Yoichi; Sawabu, Norio; Ito, Takashi.

:: Biochemical and Biophysical Research Communications, 巻 313, 番号 4, 23.01.2004, p. 922-925.

研究成果: ジャーナルへの寄稿記事

Feng, Shu Ying ; Ota, Kazuhisa ; Yamada, Yoichi ; Sawabu, Norio ; Ito, Takashi. / A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions. :: Biochemical and Biophysical Research Communications. 2004 ; 巻 313, 番号 4. pp. 922-925.
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