A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions

Shu Ying Feng, Kazuhisa Ota, Yoichi Yamada, Norio Sawabu, Takashi Ito

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

We developed a method for site-selective CpG methylation of the budding yeast genome. The method recruits LexA-fused M.SssI DNA methyltransferase to LexA operator sequences integrated adjacent to the target site. Microarray analysis of methylated DNAs indicated that the tethered enzyme selectively methylates the region around the target site. Exploiting this method to methylate bait DNA in the one-hybrid system, we demonstrated methylation-dependent DNA binding of methyl-CpG binding proteins, MBD1 and Kaiso, in vivo. This methylation-dependent one-hybrid system would provide a versatile tool for the search and analysis of proteins that recognize methylated DNA to participate in epigenetic regulation.

本文言語英語
ページ(範囲)922-925
ページ数4
ジャーナルBiochemical and Biophysical Research Communications
313
4
DOI
出版ステータス出版済み - 1 23 2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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