A yeast one-hybrid system to screen for methylated DNA-binding proteins

Shu Ying Feng, Kazuhisa Ota, Takashi Ito

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

We had previously exploited a method for targeted DNA methylation in budding yeast to succeed in one-hybrid detection of methylation-dependent DNA-protein interactions. Based on this finding, we developed a yeast one-hybrid system to screen cDNA libraries for clones encoding methylated DNA-binding proteins. Concurrent use of two independent bait sequences in the same cell, or dual-bait system, effectively reduced false positive clones, which were derived from methylationinsensitive sequence-specific DNA-binding proteins. We applied the dual-bait system to screen cDNA libraries and demonstrated efficient isolation of clones for methylated DNA-binding proteins. This system would serve as a unique research tool for epigenetics.

元の言語英語
ページ(範囲)e189
ジャーナルNucleic acids research
38
発行部数20
DOI
出版物ステータス出版済み - 11 1 2010

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DNA-Binding Proteins
Clone Cells
Yeasts
DNA Methylation
Gene Library
Saccharomycetales
Epigenomics
Research
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

A yeast one-hybrid system to screen for methylated DNA-binding proteins. / Feng, Shu Ying; Ota, Kazuhisa; Ito, Takashi.

:: Nucleic acids research, 巻 38, 番号 20, 01.11.2010, p. e189.

研究成果: ジャーナルへの寄稿記事

Feng, Shu Ying ; Ota, Kazuhisa ; Ito, Takashi. / A yeast one-hybrid system to screen for methylated DNA-binding proteins. :: Nucleic acids research. 2010 ; 巻 38, 番号 20. pp. e189.
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