A yeast one-hybrid system to screen for methylated DNA-binding proteins

Shu Ying Feng, Kazuhisa Ota, Takashi Ito

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

We had previously exploited a method for targeted DNA methylation in budding yeast to succeed in one-hybrid detection of methylation-dependent DNA-protein interactions. Based on this finding, we developed a yeast one-hybrid system to screen cDNA libraries for clones encoding methylated DNA-binding proteins. Concurrent use of two independent bait sequences in the same cell, or dual-bait system, effectively reduced false positive clones, which were derived from methylationinsensitive sequence-specific DNA-binding proteins. We applied the dual-bait system to screen cDNA libraries and demonstrated efficient isolation of clones for methylated DNA-binding proteins. This system would serve as a unique research tool for epigenetics.

本文言語英語
ページ(範囲)e189
ジャーナルNucleic acids research
38
20
DOI
出版ステータス出版済み - 11 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

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