Aberrant cell cycle checkpoint function and early embryonic death in Chk1(-/-) mice

Hiroyuki Takai, Kaoru Tominaga, Noboru Motoyama, Yohji A. Minamishima, Hiroyasu Nagahama, Tadasuke Tsukiyama, Kyoji Ikeda, Keiko Nakayama, Makoto Nakanishi, Keiichi Nakayama

研究成果: ジャーナルへの寄稿記事

410 引用 (Scopus)

抄録

The recent discovery of checkpoint kinases has suggested the conservation of checkpoint mechanisms between yeast and mammals. In yeast, the protein kinase Chk1 is thought to mediate signaling associated with the DNA damage checkpoint of the cell cycle. However, the function of Chk1 in mammals has remained unknown. Targeted disruption of Chk1 in mice showed that Chk1(-/-) embryos exhibit gross morphologic abnormalities in nuclei as early as the blastocyst stage. In culture, Chk1(-/-) blastocysts showed a severe defect in outgrowth of the inner cell mass and died of apoptosis. DNA replication block and DNA damage failed to arrest the cell cycle before initiation of mitosis in Chk1(-/-) embryos. These results may indicate that Chk1 is indispensable for cell proliferation and survival through maintaining the G2 checkpoint in mammals.

元の言語英語
ページ(範囲)1439-1447
ページ数9
ジャーナルGenes and Development
14
発行部数12
出版物ステータス出版済み - 6 15 2000

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Cell Cycle Checkpoints
Mammals
Blastocyst
DNA Damage
Embryonic Structures
Yeasts
DNA Replication
Mitosis
Cell Survival
Phosphotransferases
Cell Proliferation
Apoptosis

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

これを引用

Takai, H., Tominaga, K., Motoyama, N., Minamishima, Y. A., Nagahama, H., Tsukiyama, T., ... Nakayama, K. (2000). Aberrant cell cycle checkpoint function and early embryonic death in Chk1(-/-) mice. Genes and Development, 14(12), 1439-1447.

Aberrant cell cycle checkpoint function and early embryonic death in Chk1(-/-) mice. / Takai, Hiroyuki; Tominaga, Kaoru; Motoyama, Noboru; Minamishima, Yohji A.; Nagahama, Hiroyasu; Tsukiyama, Tadasuke; Ikeda, Kyoji; Nakayama, Keiko; Nakanishi, Makoto; Nakayama, Keiichi.

:: Genes and Development, 巻 14, 番号 12, 15.06.2000, p. 1439-1447.

研究成果: ジャーナルへの寄稿記事

Takai, H, Tominaga, K, Motoyama, N, Minamishima, YA, Nagahama, H, Tsukiyama, T, Ikeda, K, Nakayama, K, Nakanishi, M & Nakayama, K 2000, 'Aberrant cell cycle checkpoint function and early embryonic death in Chk1(-/-) mice', Genes and Development, 巻. 14, 番号 12, pp. 1439-1447.
Takai H, Tominaga K, Motoyama N, Minamishima YA, Nagahama H, Tsukiyama T その他. Aberrant cell cycle checkpoint function and early embryonic death in Chk1(-/-) mice. Genes and Development. 2000 6 15;14(12):1439-1447.
Takai, Hiroyuki ; Tominaga, Kaoru ; Motoyama, Noboru ; Minamishima, Yohji A. ; Nagahama, Hiroyasu ; Tsukiyama, Tadasuke ; Ikeda, Kyoji ; Nakayama, Keiko ; Nakanishi, Makoto ; Nakayama, Keiichi. / Aberrant cell cycle checkpoint function and early embryonic death in Chk1(-/-) mice. :: Genes and Development. 2000 ; 巻 14, 番号 12. pp. 1439-1447.
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