TY - JOUR
T1 - Aberrant structures of fecal bacterial community in allergic infants profiled by 16S rRNA gene pyrosequencing
AU - Nakayama, Jiro
AU - Kobayashi, Takako
AU - Tanaka, Shigemitsu
AU - Korenori, Yuki
AU - Tateyama, Atsushi
AU - Sakamoto, Naoshige
AU - Kiyohara, Chikako
AU - Shirakawa, Taro
AU - Sonomoto, Kenji
N1 - Funding Information:
We would like to acknowledge the financial support of Funda??o para a Ci?ncia e a Tecnologia (FCT, Portugal) through projects PTDC/QEQ-PRS/2824/2012, UID/QUI/50006/2013 (LAQV/REQUIMTE), UID/DTP/04138/2013 (iMed. ULisboa) and PEst-OE/ AGR/UI0245/2014. Carla F. Martins and Andreia Rosatella acknowledge FCT for fellowships SFRH/BD/111128/2015 and SFRH/ BPD/75045/2010, respectively.
PY - 2011/12
Y1 - 2011/12
N2 - We investigated the correlation between fecal bacteria composition in early infancy and the prevalence of allergic diseases in late infancy. The fecal microbiota in the first 2 months was profiled using the 16S rRNA V6 short-tag sequences in the community and statistically compared between two groups of subjects who did and did not show allergic symptoms in the first 2 years (n = 11 vs. 11). In the allergic group, genus Bacteroides at 1 month and genera Propionibacterium and Klebsiella at 2 months were more abundant, and genera Acinetobacter and Clostridium at 1 month were less abundant than in the nonallergic group. Allergic infants who showed high colonization of Bacteroides and/or Klebsiella showed less colonization of Clostridium perfringens/butyricum, suggesting antagonism between these bacterial groups in the gastrointestinal tract. It was also remarkable that the relative abundance of total Proteobacteria, excluding genus Klebsiella, was significantly lower in the allergic than in the nonallergic group at the age of 1 month. These results indicate that pyrosequence-based 16S rRNA gene profiling is valid to find the intestinal microbiotal disorder that correlates with allergy development in later life.
AB - We investigated the correlation between fecal bacteria composition in early infancy and the prevalence of allergic diseases in late infancy. The fecal microbiota in the first 2 months was profiled using the 16S rRNA V6 short-tag sequences in the community and statistically compared between two groups of subjects who did and did not show allergic symptoms in the first 2 years (n = 11 vs. 11). In the allergic group, genus Bacteroides at 1 month and genera Propionibacterium and Klebsiella at 2 months were more abundant, and genera Acinetobacter and Clostridium at 1 month were less abundant than in the nonallergic group. Allergic infants who showed high colonization of Bacteroides and/or Klebsiella showed less colonization of Clostridium perfringens/butyricum, suggesting antagonism between these bacterial groups in the gastrointestinal tract. It was also remarkable that the relative abundance of total Proteobacteria, excluding genus Klebsiella, was significantly lower in the allergic than in the nonallergic group at the age of 1 month. These results indicate that pyrosequence-based 16S rRNA gene profiling is valid to find the intestinal microbiotal disorder that correlates with allergy development in later life.
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U2 - 10.1111/j.1574-695X.2011.00872.x
DO - 10.1111/j.1574-695X.2011.00872.x
M3 - Article
C2 - 22029688
AN - SCOPUS:80755167698
VL - 63
SP - 397
EP - 406
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 3
ER -